Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology

• Published in: Nature immunology Vol. 24; no. 6; pp. 925 - 940
• Main Authors: Gullotta, Giorgia Serena, De Feo, Donatella, Friebel, Ekaterina, Semerano, Aurora, Scotti, Giulia Maria, Bergamaschi, Andrea, Butti, Erica, Brambilla, Elena, Genchi, Angela, Capotondo, Alessia, Gallizioli, Mattia, Coviello, Simona, Piccoli, Marco, Vigo, Tiziana, Della Valle, Patrizia, Ronchi, Paola, Comi, Giancarlo, D’Angelo, Armando, Maugeri, Norma, Roveri, Luisa, Uccelli, Antonio, Becher, Burkhard, Martino, Gianvito, Bacigaluppi, Marco
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2023; Nature Publishing Group
• Subjects: 631/250/232/2059; 631/250/256/2516; 692/420/256/2516; Aging; Animals; Biomedical and Life Sciences; Biomedicine; Blood; Geriatrics; Granulopoiesis; Hematopoietic stem cells; Immune system; Immunology; Infectious Diseases; Ischemia; Ischemic Stroke - pathology; Leukocytes; Leukocytes (neutrophilic); Mice; Neutrophils; Older people; Oxidative stress; Pathogenicity; Pathogens; Phagocytosis; Proteomes; Reperfusion; Stem cells; Stroke; Stroke - pathology
• ISSN: 1529-2908; 1529-2916
• DOI: 10.1038/s41590-023-01505-1

Aging accounts for increased risk and dismal outcome of ischemic stroke. Here, we investigated the impact of age-related changes in the immune system on stroke. Upon experimental stroke, compared with young mice, aged mice had increased neutrophil clogging of the ischemic brain microcirculation, leading to worse no-reflow and outcomes. Aged mice showed an enhanced granulopoietic response to stroke that led to the accumulation of CD101 + CD62L lo mature and CD177 hi CD101 lo CD62L lo and CD177 lo CD101 lo CD62L hi immature atypical neutrophils in the blood, endowed with increased oxidative stress, phagocytosis and procoagulant features. Production of CXCL3 by CD62L lo neutrophils of the aged had a key role in the development and pathogenicity of aging-associated neutrophils. Hematopoietic stem cell rejuvenation reverted aging-associated neutropoiesis and improved stroke outcome. In elderly patients with ischemic stroke, single-cell proteome profile of blood leukocytes identified CD62L lo neutrophil subsets associated with worse reperfusion and outcome. Our results unveil how stroke in aging leads to a dysregulated emergency granulopoiesis impacting neurological outcome. Bacigaluppi and colleagues report that the accumulation of atypical mature and immature neutrophil subsets and a dysregulated emergency granulopoiesis response in aged mice and humans affect the outcome of stroke.