Neoline, an active ingredient of the processed aconite root in Goshajinkigan formulation, targets Nav1.7 to ameliorate mechanical hyperalgesia in diabetic mice

• Published in: Journal of ethnopharmacology Vol. 259; p. 112963
• Main Authors: Nakatani, Yoshihiko, Negoro, Kanako, Yamauchi, Miki, Katasho, Maki, Ishikura, Kei-ichiro, Iwaki, Anna, Tsukada, Kazuyo, Yamaguchi, Marina, Uehara, Arata, Yoshida, Masato, Ishiuchi, Kan'ichiro, Makino, Toshiaki, Kitajima, Masaki, Ohsawa, Masahiro, Amano, Taku
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 15.09.2020
• Subjects: Aconitine - analogs & derivatives; Aconitine - isolation & purification; Aconitine - pharmacology; Aconitum - chemistry; Analgesics - isolation & purification; Analgesics - pharmacology; Animals; Behavior, Animal - drug effects; Diabetes Mellitus, Experimental - complications; Diabetic mice; Diabetic Neuropathies - etiology; Diabetic Neuropathies - metabolism; Diabetic Neuropathies - physiopathology; Diabetic Neuropathies - prevention & control; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - pharmacology; Goshajinkigan; HEK293 Cells; Humans; Hyperalgesia - etiology; Hyperalgesia - metabolism; Hyperalgesia - physiopathology; Hyperalgesia - prevention & control; Male; Membrane Potentials; Mice, Inbred ICR; Nav1.7; NAV1.7 Voltage-Gated Sodium Channel - drug effects; NAV1.7 Voltage-Gated Sodium Channel - genetics; NAV1.7 Voltage-Gated Sodium Channel - metabolism; Neoline; Pain Threshold - drug effects; Plant Roots - chemistry; Processed aconite root; Voltage-gated sodium channel; Voltage-Gated Sodium Channel Blockers - isolation & purification; Voltage-Gated Sodium Channel Blockers - pharmacology; Nav1.7; Goshajinkigan; Neoline; Processed aconite root; Diabetic mice; Voltage-gated sodium channel
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2020.112963

Goshajinkigan (GJG), a traditional Japanese Kampo formula, has been shown to exhibit several pharmacological actions, including antinociceptive effects. Processed aconite root (PA), which is considered to be an active ingredient of GJG, has also been demonstrated to have an ameliorative effect on pain, such as diabetic peripheral neuropathic pain. We recently identified neoline as the active ingredient of both GJG and PA that is responsible for its effects against oxaliplatin-induced neuropathic pain in mice. In the present study, we investigated whether GJG, PA, and neoline could inhibit Nav1.7 voltage-gated sodium channel (VGSC) current and whether neoline could ameliorate mechanical hyperalgesia in diabetic mice. To assess the electrophysiological properties of GJG extract formulation, powdered PA, and neoline on Nav1.7 VGSCs, whole-cell patch clamp recording was performed using human HEK293 cells expressing Nav1.7 VGSCs. In addition, the ameliorative effects of neoline on diabetic peripheral neuropathic pain were evaluated using the von Frey test in streptozotocin (STZ)-induced diabetic model mice. GJG extract formulation significantly inhibited Nav1.7 VGSC peak current. Powdered PA also inhibited Nav1.7 VGSC peak current. Like GJG and PA, neoline could inhibit Nav1.7 VGSC current. When diabetic mice were treated with neoline by intraperitoneal acute administration, the mechanical threshold was increased in diabetic mice, but not in non-diabetic mice, in a behavioral study. These results suggest that neoline might be a novel active ingredient of GJG and PA that is one of responsible ingredients for ameliorating mechanical hyperalgesia in diabetes via the inhibition of Nav1.7 VGSC current at least. [Display omitted]