Physico-chemical and biological properties of a nano-hydroxyapatite powder synthesized at room temperature

Abstract Objective The aim of this work was to synthesize and to characterize chemically and biologically ( in vitro and i n vivo) a nano-sized hydroxyapatite (nHA). Materials and methods Wet chemical precipitation at room temperature was performed, then chemical structure was explored using transmi...

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Published inIngénierie et recherche biomédicale Vol. 31; no. 4; pp. 226 - 233
Main Authors Catros, S, Guillemot, F, Lebraud, E, Chanseau, C, Perez, S, Bareille, R, Amédée, J, Fricain, J.C
Format Journal Article
LanguageEnglish
Published Issy-les-Moulineaux Elsevier Masson SAS 01.09.2010
Elsevier Masson
Subjects
Biological and medical sciences
Chemical Sciences
DRX
FTIR
Hydroxyapatite nano-cristalline synthétisée à basse température
In Vitro
In Vivo
Internal Medicine
Low temperature hydroxyapatite
Material chemistry
Medical sciences
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
XRD
In Vivo
FTIR
Hydroxyapatite nano-cristalline synthétisée à basse température
In Vitro
XRD
DRX
Low temperature hydroxyapatite
Biological properties
Hydroxyapatite
Nanostructure
In vitro
Powder
Osteoarticular system
In vivo
Synthesis
Osteoblast
Biomaterial
Bone
Physicochemical properties
Room temperature
Biomedical engineering
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Summary:Abstract Objective The aim of this work was to synthesize and to characterize chemically and biologically ( in vitro and i n vivo) a nano-sized hydroxyapatite (nHA). Materials and methods Wet chemical precipitation at room temperature was performed, then chemical structure was explored using transmission electron microscopy, X-ray diffraction and Fourier transformed infrared spectroscopy. In vitro biological characterization was done using MG63 osteoblastic cell line cultured onto the material, and characterization was done for morphology (scanning electron microscopy), viability (live/dead assay) and proliferation (MTT test). Finally, nHA powder was tested in vivo in a study involving C57 Black mice for bone repair in a calvarial bone critical sized defect. Results Morphological, physico-chemical and cristallographic analyses revealed specific features of hydroxyapatite. Biological in vitro experiments revealed high affinity and proliferative ability of MG63 cells cultured onto the material. In vivo study displayed that in this model, the material allowed to repair bone continuity after 1 month healing. Discussion and conclusions The different ways of nHA synthesis are discussed regarding the potential application of the material. The obtained material should find applications in bone tissue engineering experiments.
ISSN:1959-0318
DOI:10.1016/j.irbm.2010.04.002