Antibiotic resistance and molecular characterization of ophthalmic Staphylococcus pseudintermedius isolates from dogs

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 15; no. 3; pp. 409 - 415
• Main Authors: Kang, Min-Hee, Chae, Min-Joo, Yoon, Jang-Won, Kim, Seung-Gon, Lee, So-Young, Yoo, Jong-Hyun, Park, Hee-Myung
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 2014; The Korean Society of Veterinary Science
• Subjects: Animals; Anti-Bacterial Agents - therapeutic use; Dog Diseases - drug therapy; Dog Diseases - microbiology; Dogs; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Eye Infections, Bacterial - drug therapy; Eye Infections, Bacterial - microbiology; Eye Infections, Bacterial - veterinary; Microbial Sensitivity Tests - veterinary; Multiplex Polymerase Chain Reaction; Original; Staphylococcal Infections - drug therapy; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcus - drug effects; Staphylococcus - isolation & purification; 수의학; Staphylococcus pseudintermedius; methicillin resistance; canine; antibiotic resistance; ophthalmic
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2014.15.3.409

The prevalence, virulence potential, and antibiotic resistance of ophthalmic Staphylococcus pseudintermedius (SP) isolated from dogs were examined. Sixty-seven Staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the Staphylococcus intermedius group (SIG) nuclease gene (SInuc), exfoliative toxin gene for SIG (siet), and antibiotic resistance genes (blaZ and mecA). PCR-restriction fragment length polymorphism analysis of the pta gene was also performed. Fifty isolates were identified as SIG strains, all of which were found to be SP. The blaZ gene was detected in 42 of the 50 SP strains and mecA gene was observed in 18 of the 50 SP strains. The 50 SP strains were most susceptible to amoxicillin/clavulanic acid (94%) and chlorampenicol (70%), and highly resistant to tetracycline (94%) and penicillin (92%). It was also found that 16 (88.9%) mecA-positive SP strains were resistant to oxacillin, tetracycline and penicillin. All mecA-positive SP were resistant to more than four of the eight tested antibiotics and therefore considered SP with multi-drug resistance (MDR). Our results indicate a high prevalence of antibiotic resistance genes in ophthalmic SP along with a close relationship between MDR SP strains and the mecA gene. Based on our findings, judicious administration of antibiotics to companion dogs is necessary.