Stable RNA interference in Spodoptera frugiperda cells by a DNA vector-based method

• Published in: Biotechnology letters Vol. 28; no. 4; pp. 271 - 277
• Main Authors: LIN, Chih-Chien, HSU, John T.-A, HUANG, Kai-Ling, TANG, Hung-Kuan, LAI, Yiu-Kay
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.02.2006; Springer Nature B.V
• Subjects: Animals; Baculoviridae - genetics; Biological and medical sciences; Biotechnology; Deoxyribonucleic acid; DNA; DNA - administration & dosage; DNA - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Silencing - physiology; Genetic Vectors - genetics; Insecta; Luciferases, Firefly - physiology; Ribonucleic acid; RNA; RNA Interference - physiology; Spodoptera - physiology; Spodoptera frugiperda; Transfection - methods; stable RNAi; Double stranded RNA; RNA interference; Insecta; Spodoptera frugiperda; Method; double-stranded RNA; Virus; baculovirus; Baculoviridae; Arthropoda; DNA; Lepidoptera; Noctuidae; Invertebrata; Vector
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-005-5530-3

Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study.