A sensitive and accurate liquid chromatography–tandem mass spectrometry method for quantitative determination of the novel hepatitis C NS5A inhibitor BMS-790052 (daclastasvir) in human plasma and urine

• Published in: Journal of Chromatography A Vol. 1245; pp. 117 - 121
• Main Authors: Jiang, Hao, Zeng, Jianing, Kandoussi, Hamza, Liu, Yifei, Wang, Xiaodong, Bifano, Marc, Cojocaru, Laura, Ryan, John, Arnold, Mark E.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 06.07.2012
• Subjects: BMS-790052; Carbamates; Chromatography, Liquid - methods; Hepacivirus - drug effects; Hepacivirus - genetics; Hepacivirus - metabolism; hepatitis C; Hepatitis C - blood; Hepatitis C - urine; Human plasma and urine; Humans; Imidazoles - blood; Imidazoles - isolation & purification; Imidazoles - urine; ions; LC–MS/MS; liquid chromatography; Liquid-Liquid Extraction; LLE; monitoring; Pyrrolidines; quantitative analysis; Sensitivity and Specificity; tandem mass spectrometry; Tandem Mass Spectrometry - methods; urine; Valine - analogs & derivatives; Viral Nonstructural Proteins - antagonists & inhibitors; Human plasma and urine; LLE; LC–MS/MS; BMS-790052
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2012.05.028

► We developed a sensitive and accurate LC–MS/MS method with the LLOQ at 50pg/mL. ► A doubly charged precursor ion was monitored in +ESI/SRM mode, which showed a better response. ► Non-specific binding issue in sample processing was resolved by adding surfactant CHAPS. ► The validation results and sample analysis performance demonstrate the ruggedness of the method. ► The validated method provides a reference for future bioanalytical application in clinical studies. A liquid–liquid extraction (LLE) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) methods have been developed and validated for the quantification of BMS-790052 (daclastasvir) in human plasma and urine. The samples were extracted with methyl-t-butyl ether (MTBE) before analyzing by an API 4000 mass spectrometer which was operated in a multiple reaction monitoring (MRM) mode for detection of positively charged ions of BMS-790052 and its internal standard, 13C10-BMS-790052. The standard curves ranged from 0.050 to 50.0ng/mL for BMS-790052 in human plasma, and 1.00–1000ng/mL in human urine. The intra-assay precision (%CV), based on four levels of analytical QCs (low, geometric mean, mid and high), was within 8.6%; inter-assay precision (%CV) was within 6.7% for both plasma and urine methods, and the mean assay accuracy (%Dev) was within ±3.0% for both plasma and urine methods. The ruggedness of this accurate, precise, and selective LLE–LC–MS/MS method has been demonstrated in the successful analysis of several thousand clinical study samples.