Advances in detection of genetically engineered crops by multiplex polymerase chain reaction methods

In the past, several multiplex PCR (mPCR) methods have been developed and validated for precise and accurate monitoring, tracing and regulation of genetically Engineered (GE) food, feed and seed. However, a complete and updated review of mPCR is still lacking. Recently, mPCR methods have been couple...

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Bibliographic Details
Published inTrends in food science & technology Vol. 21; no. 9; pp. 442 - 454
Main Authors Shrestha, Hari K., Hwu, Kae-Kang, Chang, Men-Chi
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier Ltd 01.09.2010
Elsevier
Subjects
Biological and medical sciences
biosensors
Control
Crops
detection limit
Food industries
Foods
Fundamental and applied biological sciences. Psychology
General aspects
Genetic engineering
Germanium
Methods of analysis, processing and quality control, regulation, standards
Monitoring
Multiplexing
quantitative analysis
quantitative polymerase chain reaction
Reproducibility
transgenic plants
Genetic engineering
Control method
Analysis method
Multiplex polymerase chain reaction
Detection
Genetically modified organism
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Summary:In the past, several multiplex PCR (mPCR) methods have been developed and validated for precise and accurate monitoring, tracing and regulation of genetically Engineered (GE) food, feed and seed. However, a complete and updated review of mPCR is still lacking. Recently, mPCR methods have been coupled with different qualitative and/or quantitative methods, such as real-time PCR, micro-array, multiplex ligase chain reaction, biosensors, and microfluidic device to raise the detection limit of accuracy, sensitivity and reproducibility. In this paper, we provide thorough information and discussion of mPCR methods including their development, application and challenges for GE crops analysis.
Bibliography:http://dx.doi.org/10.1016/j.tifs.2010.06.004
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ISSN:0924-2244
1879-3053
DOI:10.1016/j.tifs.2010.06.004