Atrial natriuretic peptide and brain natriuretic peptide coexist in the secretory granules of human cardiac myocytes

To elucidate the intracellular localization of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in human cardiac myocytes, an immunocytochemical study was carried out by a double immunogold technique using antisera highly specific for ANP and BNP. Surgical and autoptic tissue spe...

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Published inAmerican journal of hypertension Vol. 4; no. 11; p. 909
Main Authors Nakamura, S, Naruse, M, Naruse, K, Kawana, M, Nishikawa, T, Hosoda, S, Tanaka, I, Yoshimi, T, Yoshihara, I, Inagami, T
Format Journal Article
LanguageEnglish
Published United States 01.11.1991
Subjects
Atrial Natriuretic Factor - analysis
Cytoplasmic Granules - chemistry
Cytoplasmic Granules - ultrastructure
Humans
Immunohistochemistry - methods
Microscopy, Electron
Myocardium - chemistry
Myocardium - cytology
Myocardium - ultrastructure
Natriuretic Peptide, Brain
Nerve Tissue Proteins - analysis
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Summary:To elucidate the intracellular localization of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) in human cardiac myocytes, an immunocytochemical study was carried out by a double immunogold technique using antisera highly specific for ANP and BNP. Surgical and autoptic tissue specimens of human heart were studied. In the atrial myocytes, ANP was localized in almost all of the secretory granules, whereas BNP was colocalized with ANP in some of the granules. Although very few secretory granules were observed in ventricular myocytes, colocalization of ANP and BNP was basically the same as in atrial myocytes. No immunoreactive products were found in the control studies. These results suggest that secretion of BNP is under a regulatory mechanism similar to that of ANP.
ISSN:0895-7061
DOI:10.1093/ajh/4.11.909