Antithrombin III prevents deleterious effects of remote ischemia-reperfusion injury on healing of colonic anastomoses
Background: Antithrombin III is known as the most important natural inhibitor of thrombin activity and has been shown to attenuate local harmful effects of ischemia-reperfusion injury in many organs. In recent animal studies, delaying effect of remote organ ischemia-reperfusion injury on healing of...
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Published in | The American journal of surgery Vol. 184; no. 2; pp. 160 - 165 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Elsevier Inc
01.08.2002
Elsevier Elsevier Limited |
Subjects | |
Online Access | Get full text |
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Summary: | Background: Antithrombin III is known as the most important natural inhibitor of thrombin activity and has been shown to attenuate local harmful effects of ischemia-reperfusion injury in many organs. In recent animal studies, delaying effect of remote organ ischemia-reperfusion injury on healing of intestinal anastomoses has been demonstrated. In this study, we investigated whether antithrombin III reduces deleterious systemic effects of ischemia-reperfusion injury on healing of colonic anastomoses in rats.
Methods: Anastomosis of the left colon was performed in 24 rats that were divided into three groups: sham operated control (group I, n = 8), 30 minutes of intestinal ischemia-reperfusion by superior mesenteric artery occlusion (group II, n = 8), antithrombin III treated group (250 U/kg before and after the ischemia-reperfusion, group III, n = 8). On postoperative day 6, all animals were sacrificed, and bursting pressure and tissue hydroxyproline content of the anastomoses were assessed and compared.
Results: On postoperative day 6 the mean bursting pressures were 149.6 ± 4.8, 69.8 ± 13.5, and 121.8 ± 8.7 mm Hg for groups I, II, and III, respectively (
P = 0.000). Mean tissue hydroxyproline concentration values were 389.5 ± 29.6, 263.1 ± 10.0, and 376.0 ± 33.8 μg/mg for groups I, II, III respectively (
P = 0.005).
Conclusions: This study showed that, antithrombin III treatment significantly prevented the delaying effect of remote organ ischemia-reperfusion injury on anastomotic healing in the colon. Further clinical studies are needed to clarify whether antithrombin may be a useful therapeutic agent to increase the safety of the anastomosis during particular operations where remote organ ischemia-reperfusion injury takes place. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0002-9610 1879-1883 |
DOI: | 10.1016/S0002-9610(02)00908-X |