Effect of temperature on proton efflux from isolated chloroplast thylakoids [Mung beans, barley, peas]

• Published in: Plant physiology (Bethesda) Vol. 67; no. 6; pp. 1259 - 1263
• Main Author: Nolan, W.G
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Physiologists 01.06.1981
• Subjects: Barley; Cell membranes; Chloroplasts; Hordeum; Leguminosae; Lipids; Peas; Plants; Protons; Spinach; temperature; Temperature dependence; Thylakoids; Vigna radiata
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.67.6.1259

Temperature-induced changes in the decay of the light-induced proton gradient of chloroplast thylakoids isolated from chilling-resistant and chilling-sensitive plants have been examined. In the presence of N-methylphenazonium methosulfate, the thylakoids isolated from chilling-resistant barley (cv. Kanby) and pea (cv. Alaska) and chilling-sensitive mung bean (cv. Berken) plants showed temperature-induced changes at approximately 8.6, 13.3, and 14.0 C, respectively. Barley thylakoids assayed in the presence of sodium thiocyanate also showed a change at 8.6 C, whereas with no addition or upon the inclusion of both N-methylphenazonium methosulfate and sodium thiocyanate the change occurred at approximately 11.5 C. Temperature-induced changes in electron transport activities occurred at temperatures approximating those for proton efflux for each of the three plants. These results indicate that temperature has a general effect on thylakoid membranes and that chloroplast thylakoids from chilling-resistant and chilling-sensitive plants have their proton permeability properties affected similarly by temperature.