A paper-based nanomodified electrochemical biosensor for ethanol detection in beers

• Published in: Analytica chimica acta Vol. 960; pp. 123 - 130
• Main Authors: Cinti, Stefano, Basso, Mattia, Moscone, Danila, Arduini, Fabiana
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2017
• Subjects: Alcohol Oxidoreductases - chemistry; Alcohol Oxidoreductases - metabolism; Beer - analysis; Biosensing Techniques - instrumentation; Carbon black; Disposable Equipment; Electrochemistry; Electrodes; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Ethanol - analysis; Ethanol oxidase; Limit of Detection; Nanotechnology - instrumentation; Paper-based screen-printed electrode; Pichia - enzymology; Prussian blue nanoparticles; Wax printing; Prussian blue nanoparticles; Paper-based screen-printed electrode; Wax printing; Carbon black; Ethanol oxidase
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2017.01.010

Herein, we report the first example of a paper-based screen-printed biosensor for the detection of ethanol in beer samples. Common office paper was adopted to fabricate the analytical device. The properties of this paper-based screen-printed electrode (SPE) were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy, and they were compared with the well-established polyester-based SPEs as well. Paper demonstrated similar properties when compared with polyester, highlighting suitability towards its utilization in sensor development, with the advantages of low cost and simple disposal by incineration. A nanocomposite formed by Carbon Black (CB) and Prussian Blue nanoparticles (PBNPs), namely CB/PBNPs, was utilized as an electrocatalyst to detect the hydrogen peroxide generated by the enzymatic reaction between alcohol oxidase (AOx) and ethanol. After optimizing the analytical parameters, such as pH, enzyme, concentration, and working potential, the developed biosensor allowed a facile quantification of ethanol up to 10 mM (0.058 %vol), with a sensitivity of 9.13 μA/mM cm2 (1574 μA/%vol cm2) and a detection limit equal to 0.52 mM (0.003%vol). These satisfactory performances rendered the realized paper-based biosensor reliable over the analysis of ethanol contained in four different types of beers, including Pilsner, Weiss, Lager, and alcohol-free. The proposed manufacturing approach offers an affordable and sustainable tool for food quality control and for the realization of different electrochemical sensors and biosensors as well. [Display omitted] •Novel ethanol biosensor fabricated onto office paper.•Enhanced hydrogen peroxide detection using Carbon black/Prussian blue nanoparticles.•Only 100 μL required to perform measurements.•Paper-based electrochemical device coupled with a portable potentiostat.•Rapid quantification of ethanol in beer samples.