Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera

• Published in: Neuroscience letters Vol. 359; no. 3; pp. 190 - 194
• Main Authors: Funada, Masahiro, Yasuo, Shinobu, Yoshimura, Takashi, Ebihara, Shizufumi, Sasagawa, Hiromi, Kitagawa, Yasuo, Kadowaki, Tatsuhiko
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 15.04.2004; Elsevier
• Subjects: Amino Acid Sequence; Animals; Apidae; Apis mellifera; Bees - chemistry; Bees - genetics; Bees - metabolism; Behavior; Biological and medical sciences; Brain - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; Gene Expression Regulation; Honeybee; l-Glutamate; Metabotropic glutamate receptor; Molecular Sequence Data; Mushroom body; Organ Specificity; Phylogeny; Receptors, Metabotropic Glutamate - chemistry; Receptors, Metabotropic Glutamate - classification; Receptors, Metabotropic Glutamate - genetics; Receptors, Metabotropic Glutamate - metabolism; Vertebrates: nervous system and sense organs; l-Glutamate; Honeybee; Behavior; Mushroom body; Metabotropic glutamate receptor; Insecta; Apidae; Glutamate receptor; Glutamate; Apis mellifera; Characterization; Metabotropic receptor; Apoidea; Arthropoda; Excitatory aminoacid; Neurotransmitter; Hymenoptera; Invertebrata; Aculeata
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2004.02.004

l-Glutamate is a major neurotransmitter at the excitatory synapses in the vertebrate brain. It is also the excitatory neurotransmitter at neuromuscular junctions in insects, however its functions in their brains remain to be established. We identified and characterized two different subtypes (AmGluRA and AmGluRB) of metabotropic glutamate receptors (mGluRs) from an eusocial insect, honeybee. Both AmGluRA and AmGluRB form homodimers independently on disulfide bonds, and bind [ 3H]glutamate with K D values of 156.7 and 80.7 nM, respectively. AmGluRB is specifically expressed in the brain, while AmGluRA is expressed in the brain and other body parts, suggesting that AmGluRA is also present at the neuromuscular junctions. Both mGluRs are expressed in the mushroom bodies and the brain regions of honeybees, where motor neurons are clustered. Their expression in the brain apparently overlaps, suggesting that they may interact with each other to modulate the glutamatergic neurotransmission.