Regioselectivity of new bacterial lipases determined by hydrolysis of triolein
The newly identified lipases of 67 bacterial strains, primarily Bacillus and Pseudomonas, from the ARS Culture Collection have been characterized on the basis of their positional specificity for triglycerides (triolein). Lipase was produced by growing the cultures in tryptone-glucose-yeast extract m...
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Published in | Current microbiology Vol. 44; no. 5; pp. 336 - 340 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Springer
01.05.2002
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | The newly identified lipases of 67 bacterial strains, primarily Bacillus and Pseudomonas, from the ARS Culture Collection have been characterized on the basis of their positional specificity for triglycerides (triolein). Lipase was produced by growing the cultures in tryptone-glucose-yeast extract medium for 24 h at 30 degrees C before addition of triglyceride. The lipase was allowed to act on the triglyceride for 3 days before analysis by thin-layer chromatography. Of the bacterial lipases tested, 55 displayed random specificity, 9 were 1,3-specific, and 3 showed no apparent lipase activity under these conditions. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0343-8651 1432-0991 |
DOI: | 10.1007/s00284-001-0019-3 |