The fluorescence and resonance Rayleigh scattering spectral study and analytical application of cerium (IV) and cefoperazone system

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 162; pp. 93 - 97
• Main Authors: Yuan, Yusheng, Fu, Shenghui, Xu, Qianying, Yang, Jidong, Hu, Xiaoli, Liu, Shaopu
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.06.2016
• Subjects: Cefoperazone; Cefoperazone - chemistry; Cerium (IV); Cerium - chemistry; Fluorescence; Hydrogen-Ion Concentration; Resonance Rayleigh scattering; Scattering, Radiation; Spectrometry, Fluorescence; Fluorescence; Cefoperazone; Cerium (IV); Resonance Rayleigh scattering
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2016.03.001

In weak acidic medium of pH3.5–5.6, Ce(IV) can be reduced by cefoperazone (CPZ) to be Ce(III), which further combined with CPZ to form complex Ce(OH)3CPZ. This complex not only has higher fluorescence than Ce(III), but also results in significant increase of resonance Rayleigh scattering (RRS), second order scattering (SOS) and frequency doubling scattering (FDS). The wavelengths of maximum fluorescence exciting and emission are located at 356nm/349nm, while the maximum wavelengths of RRS, SOS and FDS are at 312nm, 550nm and 390nm, respectively. The intensity of fluorescence and scattering are all linear with the concentration of CPZ in certain conditions. The detection limit of most sensitive RRS method for CPZ is 2.1ngmL−1. The optimum conditions for detecting CPZ using RRS method are investigated. The effect of co-existing substances shows that the method has excellent selectivity, especially since other cephalosporins don't have similar reactions. Therefore, it can be achieved to determine CPZ in cephalosporins selectively. The paper also focuses on the reaction mechanism, the consistent and contracture of the resultant. The reasons for enhanced intensity are presumed in the meantime. Florescence spectra. 1: Ce(IV); 2: Ce(IV)–CPZ; cCe(IV)=3.0×10−5molL−1; c(CPZ)=2.0μgmL−1. [Display omitted] •A simple, fast method for the determination of cefoperazone is performed.•The mechanism aspect concerning the remarkable fluorescence response was discussed.•The validity was verified by comparing the results with other methods.