High-dimensional intravital microscopy reveals major changes in splenic immune system during postnatal development

Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen leukocytes evolve across the developmental phase, and how they spatially organize and interact is still poorly understood. Using a novel combination...

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Published inFrontiers in immunology Vol. 13; p. 1002919
Main Authors Porto-Pedrosa, Maria Luiza Mundim, de Miranda, Camila Dutra Moreira, Lopes, Mateus Eustáquio, Nakagaki, Brenda Naemi, Mafra, Kassiana, de Paula, Cristina Maria Pinto, Diniz, Ariane Barros, Costa, Karen Marques de Oliveira, Antunes, Maisa Mota, Oliveira, André Gustavo, Balderas, Robert, Lopes, Rodrigo Pestana, Menezes, Gustavo Batista
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 01.12.2022
Subjects
Adult
B-Lymphocytes
development
Humans
Immunology
in vivo imaging
Infant, Newborn
Intravital Microscopy
Lymphocytes
Malaria
Spleen
development
in vivo imaging
malaria
spleen
immunology
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Abstract Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen leukocytes evolve across the developmental phase, and how they spatially organize and interact is still poorly understood. Using a novel combination of selected antibodies and fluorophores to image in vivo the spleen immune environment, we described for the first time the dynamics of immune development across postnatal period. We found that spleens from adults and infants had similar numbers and arrangement of lymphoid cells. In contrast, splenic immune environment in newborns is sharply different from adults in almost all parameters analysed. Using this in vivo approach, B cells were the most frequent subtype throughout the development. Also, we revealed how infections - using a model of malaria - can change the spleen immune profile in adults and infants, which could become the key to understanding different severity grades of infection. Our new imaging solutions can be extremely useful for different groups in all areas of biological investigation, paving a way for new intravital approaches and advances.
AbstractList Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen leukocytes evolve across the developmental phase, and how they spatially organize and interact in vivo is still poorly understood. Using a novel combination of selected antibodies and fluorophores to image in vivo the spleen immune environment, we described for the first time the dynamics of immune development across postnatal period. We found that spleens from adults and infants had similar numbers and arrangement of lymphoid cells. In contrast, splenic immune environment in newborns is sharply different from adults in almost all parameters analysed. Using this in vivo approach, B cells were the most frequent subtype throughout the development. Also, we revealed how infections – using a model of malaria - can change the spleen immune profile in adults and infants, which could become the key to understanding different severity grades of infection. Our new imaging solutions can be extremely useful for different groups in all areas of biological investigation, paving a way for new intravital approaches and advances.
Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen leukocytes evolve across the developmental phase, and how they spatially organize and interact in vivo is still poorly understood. Using a novel combination of selected antibodies and fluorophores to image in vivo the spleen immune environment, we described for the first time the dynamics of immune development across postnatal period. We found that spleens from adults and infants had similar numbers and arrangement of lymphoid cells. In contrast, splenic immune environment in newborns is sharply different from adults in almost all parameters analysed. Using this in vivo approach, B cells were the most frequent subtype throughout the development. Also, we revealed how infections – using a model of malaria - can change the spleen immune profile in adults and infants, which could become the key to understanding different severity grades of infection. Our new imaging solutions can be extremely useful for different groups in all areas of biological investigation, paving a way for new intravital approaches and advances.
Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen leukocytes evolve across the developmental phase, and how they spatially organize and interact is still poorly understood. Using a novel combination of selected antibodies and fluorophores to image in vivo the spleen immune environment, we described for the first time the dynamics of immune development across postnatal period. We found that spleens from adults and infants had similar numbers and arrangement of lymphoid cells. In contrast, splenic immune environment in newborns is sharply different from adults in almost all parameters analysed. Using this in vivo approach, B cells were the most frequent subtype throughout the development. Also, we revealed how infections - using a model of malaria - can change the spleen immune profile in adults and infants, which could become the key to understanding different severity grades of infection. Our new imaging solutions can be extremely useful for different groups in all areas of biological investigation, paving a way for new intravital approaches and advances.
Author Costa, Karen Marques de Oliveira
de Miranda, Camila Dutra Moreira
Menezes, Gustavo Batista
Nakagaki, Brenda Naemi
Porto-Pedrosa, Maria Luiza Mundim
de Paula, Cristina Maria Pinto
Mafra, Kassiana
Diniz, Ariane Barros
Lopes, Rodrigo Pestana
Antunes, Maisa Mota
Oliveira, André Gustavo
Balderas, Robert
Lopes, Mateus Eustáquio
AuthorAffiliation 4 BD Biosciences, Department of Medical & Scientific Affairs, São Paulo , São Paulo , Brazil
1 Center for Gastrointestinal Biology, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil
2 Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil
3 BD Biosciences, Department of Biological Sciences , San Jose, CA , United States
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– name: 2 Departamento de Fisiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil
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  givenname: Cristina Maria Pinto
  surname: de Paula
  fullname: de Paula, Cristina Maria Pinto
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– sequence: 7
  givenname: Ariane Barros
  surname: Diniz
  fullname: Diniz, Ariane Barros
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  givenname: Maisa Mota
  surname: Antunes
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  givenname: André Gustavo
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  givenname: Gustavo Batista
  surname: Menezes
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  organization: Center for Gastrointestinal Biology, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
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Keywords development
in vivo imaging
malaria
spleen
immunology
Language English
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Edited by: Heitor Affonso Paula Neto, Federal University of Rio de Janeiro, Brazil
Reviewed by: Innocent Safeukui, University of Notre Dame, United States; Juliana Pavan Zuliani, Oswaldo Cruz Foundation (Fiocruz), Brazil
This article was submitted to B Cell Biology, a section of the journal Frontiers in Immunology
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    fullname: Bansal
– volume: 155
  start-page: 395
  year: 2020
  ident: B10
  article-title: Update on malaria
  publication-title: Med Clín (English Ed)
  doi: 10.1016/j.medcle.2020.05.024
  contributor:
    fullname: Varo
– volume: 5
  year: 2005
  ident: B3
  article-title: Structure and function of the spleen
  publication-title: Nat Rev Immunol
  doi: 10.1038/nri1669
  contributor:
    fullname: Mebius
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Snippet Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen...
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SubjectTerms Adult
B-Lymphocytes
development
Humans
Immunology
in vivo imaging
Infant, Newborn
Intravital Microscopy
Lymphocytes
Malaria
Spleen
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Title High-dimensional intravital microscopy reveals major changes in splenic immune system during postnatal development
URI https://www.ncbi.nlm.nih.gov/pubmed/36531990
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Volume 13
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