High-dimensional intravital microscopy reveals major changes in splenic immune system during postnatal development

Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen leukocytes evolve across the developmental phase, and how they spatially organize and interact is still poorly understood. Using a novel combination...

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Published inFrontiers in immunology Vol. 13; p. 1002919
Main Authors Porto-Pedrosa, Maria Luiza Mundim, de Miranda, Camila Dutra Moreira, Lopes, Mateus Eustáquio, Nakagaki, Brenda Naemi, Mafra, Kassiana, de Paula, Cristina Maria Pinto, Diniz, Ariane Barros, Costa, Karen Marques de Oliveira, Antunes, Maisa Mota, Oliveira, André Gustavo, Balderas, Robert, Lopes, Rodrigo Pestana, Menezes, Gustavo Batista
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 01.12.2022
Subjects
Adult
B-Lymphocytes
development
Humans
Immunology
in vivo imaging
Infant, Newborn
Intravital Microscopy
Lymphocytes
Malaria
Spleen
development
in vivo imaging
malaria
spleen
immunology
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Summary:Spleen is a key organ for immunologic surveillance, acting as a firewall for antigens and parasites that spread through the blood. However, how spleen leukocytes evolve across the developmental phase, and how they spatially organize and interact is still poorly understood. Using a novel combination of selected antibodies and fluorophores to image in vivo the spleen immune environment, we described for the first time the dynamics of immune development across postnatal period. We found that spleens from adults and infants had similar numbers and arrangement of lymphoid cells. In contrast, splenic immune environment in newborns is sharply different from adults in almost all parameters analysed. Using this in vivo approach, B cells were the most frequent subtype throughout the development. Also, we revealed how infections - using a model of malaria - can change the spleen immune profile in adults and infants, which could become the key to understanding different severity grades of infection. Our new imaging solutions can be extremely useful for different groups in all areas of biological investigation, paving a way for new intravital approaches and advances.
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Edited by: Heitor Affonso Paula Neto, Federal University of Rio de Janeiro, Brazil
Reviewed by: Innocent Safeukui, University of Notre Dame, United States; Juliana Pavan Zuliani, Oswaldo Cruz Foundation (Fiocruz), Brazil
This article was submitted to B Cell Biology, a section of the journal Frontiers in Immunology
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2022.1002919