Induction of rapid and reversible cytokeratin filament network remodeling by inhibition of tyrosine phosphatases

• Published in: Journal of cell science Vol. 115; no. Pt 21; pp. 4133 - 4148
• Main Authors: Strnad, Pavel, Windoffer, Reinhard, Leube, Rudolf E
• Format: Journal Article
• Language: English
• Published: England 01.11.2002
• Subjects: 14-3-3 Proteins; Cytoplasmic Granules - drug effects; Cytoplasmic Granules - metabolism; Cytoplasmic Granules - ultrastructure; Enzyme Inhibitors - pharmacology; Eukaryotic Cells - enzymology; Eukaryotic Cells - ultrastructure; Fluorescent Antibody Technique; Green Fluorescent Proteins; Intermediate Filament Proteins - metabolism; Intermediate Filaments - genetics; Intermediate Filaments - metabolism; Intermediate Filaments - ultrastructure; Keratins - genetics; Keratins - metabolism; Keratins - ultrastructure; Luminescent Proteins; Microscopy, Electron; Phosphorylation - drug effects; Plectin; Protein Tyrosine Phosphatases - antagonists & inhibitors; Protein Tyrosine Phosphatases - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Tumor Cells, Cultured; Tyrosine - metabolism; Tyrosine 3-Monooxygenase - metabolism; Vanadates - pharmacology

The cytokeratin filament network is intrinsically dynamic, continuously exchanging subunits over its entire surface, while conferring structural stability on epithelial cells. However, it is not known how cytokeratin filaments are remodeled in situations where the network is temporarily and spatiall...