Characterization of pneumococcal serotype 7F in vaccine conjugation

Streptococcus pneumoniae is a highly invasive bacterial pathogen that can cause a range of illnesses. Pneumococcal capsular polysaccharides (CPS) are the main virulence factors that causes invasive pneumococcal disease (IPD). Pneumococcal CPS serotype 7F along with a few other serotypes is more inva...

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Bibliographic Details
Published inGlycoconjugate journal Vol. 40; no. 5; pp. 565 - 573
Main Authors Deng, James Z, Jia, Xiujuan, Zong, Chengli, He, Jian, Ha, Sha, Zhuang, Ping
Format Journal Article
LanguageEnglish
Published New York Springer US 01.10.2023
Springer Nature B.V
Subjects
Biochemistry
Biomedical and Life Sciences
Capsular polysaccharides
Chromatography
Conformation
Life Sciences
Light scattering
Liquid chromatography
Monosaccharides
Pathology
Serotypes
Streptococcus infections
Vaccine development
Vaccines
Virulence factors
Ultra-performance liquid chromatography (UPLC)
Pneumococcal conjugate vaccine
Multi-angle light scattering (MALS)
Pneumococcal capsular polysaccharide 7F
Dynamic light scattering (DLS)
Size-exclusion chromatography (SEC)
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Summary:Streptococcus pneumoniae is a highly invasive bacterial pathogen that can cause a range of illnesses. Pneumococcal capsular polysaccharides (CPS) are the main virulence factors that causes invasive pneumococcal disease (IPD). Pneumococcal CPS serotype 7F along with a few other serotypes is more invasive and likely to cause IPD. Therefore, 7F is a target for pneumococcal vaccine development, and is included in the two recently approved multi-valent pneumococcal conjugated vaccines, i.e. VAXNEUVANCE and PREVNAR 20. To support process and development of our 15-valent pneumococcal conjugated vaccine (PCV15), chromatographic methods have been developed for 7F polysaccharide and conjugate characterization. A size-exclusion chromatography (SEC) method with UV, light scattering and refractive index detections was employed for concentration, size and conformation analysis. A reversed-phase ultra-performance liquid chromatography (RP-UPLC) method was used for analysis of conjugate monosaccharide composition and degree of conjugation. The collective information obtained by these chromatographic analysis provided insights into the pneumococcal conjugate and conjugation process. Graphical Abstract
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ISSN:0282-0080
1573-4986
1573-4986
DOI:10.1007/s10719-023-10125-8