Laccase–gold nanoparticle assisted bioelectrocatalytic reduction of oxygen

• Published in: Electrochemistry communications Vol. 12; no. 7; pp. 933 - 935
• Main Authors: Dagys, Marius, Haberska, Karolina, Shleev, Sergey, Arnebrant, Thomas, Kulys, Juozas, Ruzgas, Tautgirdas
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.07.2010; Amsterdam Elsevier; New York, NY
• Subjects: Bioelectrocatalysis; BIOFUEL CELLS; Biological and medical sciences; DIRECT ELECTRON-TRANSFER; Electrochemistry; ENZYMES; Fundamental and applied biological sciences. Psychology; Gold nanoparticle; Laccase; Molecular biophysics; Oxygen reduction; Physical chemistry in biology; REDOX; Oxygen reduction; Gold nanoparticle; Bioelectrocatalysis; Laccase; Gold; Oxygen; Enzyme; Nanoparticle; Bioelectrochemistry; Electron transfer; Enzymatic catalysis; Transition metal; Carbon; Electrodes; Chemical reduction; Electrocatalysis; Oxidoreductases; Electrochemical reaction; Glassy state; Modified material
• ISSN: 1388-2481; 1873-1902; 1873-1902
• DOI: 10.1016/j.elecom.2010.04.024

It was found that homogeneous activity of Trametes hirsuta laccase is considerably diminished in the presence of gold nanoparticles (Au-NPs). Heterogeneous electron transfer studies revealed that Au-NPs facilitate direct electron transfer (DET) between the T1 copper site of the laccase and the surface of Au-NP modified electrodes. DET was characterized by the standard heterogeneous ET constant of 0.5 ± 0.6 s − 1 at Au-NPs with an average diameter of 50 nm. As a consequence of this a well pronounced DET based bioelectrocatalytic oxygen reduction with current densities of 5–30 µA cm − 2 has been achieved at the laccase–Au-NP modified electrodes.