Streptococcus mutans and Caries: A Systematic Review and Meta-Analysis

• Published in: Journal of dental research Vol. 104; no. 6; pp. 594 - 603
• Main Authors: Mazurel, D., Brandt, B.W., Boomsma, M., Crielaard, W., Lagerweij, M., Exterkate, R.A.M., Deng, D.M.
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA SAGE Publications 01.06.2025; SAGE PUBLICATIONS, INC
• Subjects: Dental caries; Dental Caries - epidemiology; Dental Caries - microbiology; Humans; Meta-analysis; Microbiomes; Microbiota; Mouth - microbiology; Prevalence; Relative abundance; Reviews; Streptococcus mutans; Streptococcus mutans - isolation & purification; microbiota; saliva; dental plaque; high-throughput nucleotide sequencing; 16s ribosomal rna; real-time polymerase chain reaction
• ISSN: 0022-0345; 1544-0591; 1544-0591
• DOI: 10.1177/00220345241303880

It has been questioned whether Streptococcus mutans can still be considered the major etiological agent for caries. The main argument is that most evidence has been based on single-species identification. The composition of the oral microbiome was not analyzed. This systemic review aims to assess the prevalence and abundance of S. mutans in caries-active (CA) and caries-free (CF) subjects based on clinical studies in which the microbiome was investigated. Three databases (PubMed, Cochrane, Embase) were searched until May 22, 2023, for eligible publications that included CA and CF subjects and reported the detection of both S. mutans and the oral microbial community, using DNA-based methods. The clinical and microbial outcomes were summarized and further analyzed using a random-effects model. Of 22 eligible studies, 3 were excluded due to the high risk of bias. In the remaining 19 studies, 16 reported the prevalence of S. mutans, 11 reported its relative abundance, and 8 reported both parameters. The prevalence of S. mutans in CA was either similar to (n = 4) or higher than (n = 12) the CF group. The reported relative abundance in CA was higher than CF in all 11 studies, although the values varied from 0.001% to 5%. Meta-analysis confirmed the significance of these findings. The summary of microbial community data did not reveal other caries-associated bacterial genera/species than S. mutans. In conclusion, the collected evidence based on microbiome studies suggests a strong association between the prevalence and abundance of S. mutans and caries experience. While the cariogenic role of S. mutans in the oral ecosystem should be recognized, its actual function warrants further exploration.