Diagnostic methods for neonatal hyperbilirubinemia: benefits, limitations, requirements, and novel developments

• Published in: Pediatric research Vol. 90; no. 2; pp. 277 - 283
• Main Authors: Hulzebos, Christian V., Vitek, Libor, Coda Zabetta, Carlos D., Dvořák, Aleš, Schenk, Paul, van der Hagen, Eline A. E., Cobbaert, Christa, Tiribelli, Claudio
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.08.2021; Nature Publishing Group
• Subjects: Bilirubin - blood; Biomarkers - blood; Humans; Hyperbilirubinemia, Neonatal - blood; Hyperbilirubinemia, Neonatal - diagnosis; Hyperbilirubinemia, Neonatal - therapy; Infant, Newborn; Jaundice; Jaundice, Neonatal - blood; Jaundice, Neonatal - diagnosis; Jaundice, Neonatal - therapy; Medical diagnosis; Medicine; Medicine & Public Health; Neonatal Screening; Newborn babies; Pediatric Surgery; Pediatrics; Point-of-Care Testing; Predictive Value of Tests; Prognosis; Reproducibility of Results; Review Article; Severity of Illness Index; Up-Regulation
• ISSN: 0031-3998; 1530-0447
• DOI: 10.1038/s41390-021-01546-y

Invasive bilirubin measurements remain the gold standard for the diagnosis and treatment of infants with severe neonatal hyperbilirubinemia. The present paper describes different methods currently available to assess hyperbilirubinemia in newborn infants. Novel point-of-care bilirubin measurement methods, such as the BiliSpec and the Bilistick, would benefit many newborn infants, especially in low-income and middle-income countries where the access to costly multi-analyzer in vitro diagnostic instruments is limited. Total serum bilirubin test results should be accurate within permissible limits of measurement uncertainty to be fit for clinical purposes. This implies correct implementation of internationally endorsed reference measurement systems as well as participation in external quality assessment programs. Novel analytic methods may, apart from bilirubin, include the determination of bilirubin photoisomers and bilirubin oxidation products in blood and even in other biological matrices. Impact Key message: Bilirubin measurements in blood remain the gold standard for diagnosis and treatment of severe neonatal hyperbilirubinemia (SNH). External quality assessment (EQA) plays an important role in revealing inaccuracies in diagnostic bilirubin measurements. What does this article add to the existing literature? We provide analytic performance data on total serum bilirubin (TSB) as measured during recent EQA surveys. We review novel diagnostic point-of-care (POC) bilirubin measurement methods and analytic methods for determining bilirubin levels in biological matrices other than blood. Impact: Manufacturers should make TSB test results traceable to the internationally endorsed total bilirubin reference measurement system and should ensure permissible limits of measurement uncertainty.