Catenisphaera adipataccumulans gen. nov., sp. nov., a member of the family Erysipelotrichaceae isolated from an anaerobic digester

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. Pt 3; pp. 805 - 810
• Main Authors: Kanno, Manabu, Katayama, Taiki, Morita, Naoki, Tamaki, Hideyuki, Hanada, Satoshi, Kamagata, Yoichi
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.03.2015
• Subjects: alanine; anaerobic digesters; bacteria; Bacteria, Anaerobic - classification; Bacteria, Anaerobic - genetics; Bacteria, Anaerobic - isolation & purification; Bacterial Typing Techniques; Base Composition; Bioreactors - microbiology; chemotaxonomy; DNA; DNA, Bacterial - genetics; fatty acids; Fatty Acids - chemistry; Fermentation; Firmicutes; galactose; glucose; glutamic acid; Gram-Positive Rods - classification; Gram-Positive Rods - genetics; Gram-Positive Rods - isolation & purification; Japan; lysine; Molecular Sequence Data; nucleotide sequences; peptidoglycans; phospholipids; Phospholipids - chemistry; Phylogeny; rhamnose; ribose; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; sequence homology; serine; threonine; yeast extract; Japan
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.000021

An obligately anaerobic bacterium, designated strain GK12 ᵀ, was isolated from an anaerobic digester in Fukagawa, Hokkaido Prefecture, Japan. The cells of strain GK12 ᵀ were non-motile, non-spore-forming cocci that commonly occurred in chains. 16S rRNA gene sequence analysis revealed that strain GK12 ᵀ was affiliated with the family Erysipelotrichaceae in the phylum Firmicutes and showed 91.8 % sequence similarity to the most closely related species, Faecalicoccus acidiformans . The strain grew at 30–50 °C (optimally at 40 °C) and at pH 5.5–8.5 (optimally at pH 7.5). The main end product of glucose fermentation was lactate. Yeast extract was required for growth. The strain contained C ₁₄ : ₀, C ₁₄ : ₀ 1,1-dimethoxyalkane (DMA), C ₁₆ : ₀ DMA and C ₁₈ : ₀ DMA as the major cellular fatty acids (>10 % of the total). The polar lipid profile was composed of phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The whole-cell sugars were galactose, rhamnose and ribose. The cell-wall murein contained alanine, glutamic acid, lysine, serine and threonine, but not diaminopimelic acid. The G+C content of the genomic DNA was 47.7 mol%. Based on phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Catenisphaera adipataccumulans gen. nov., sp. nov., is proposed to accommodate strain GK12 ᵀ (= NBRC 108915 ᵀ = DSM 25799 ᵀ).