A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

A novel electrochemical sensing strategy was developed for highly sensitive and specific detection of DNA by combining the exonuclease III-assisted target recycling amplification with mismatched catalytic hairpin assembly. The proposed method showed high sensitivity and selectivity with a dynamic re...

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Published inChemical communications (Cambridge, England) Vol. 51; no. 20; pp. 4220 - 4222
Main Authors Tao, Chenyang, Yan, Yurong, Xiang, Hua, Zhu, Dan, Cheng, Wei, Ju, Huangxian, Ding, Shijia
Format Journal Article
LanguageEnglish
Published England 11.03.2015
Subjects
Amplification
Assembly
Biosensing Techniques
Catalysis
Catalysts
Deoxyribonucleic acid
DNA - analysis
Dynamic response
Electrochemical Techniques
Exodeoxyribonucleases - chemistry
Nucleic Acid Amplification Techniques
Recycling
Strategy
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Summary:A novel electrochemical sensing strategy was developed for highly sensitive and specific detection of DNA by combining the exonuclease III-assisted target recycling amplification with mismatched catalytic hairpin assembly. The proposed method showed high sensitivity and selectivity with a dynamic response range from 100 fM to 5 nM.
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ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc00385g