In-Vivo Tape Stripping Study with Caffeine for Comparisons on Body Sites, Age and Washing

• Published in: Pharmaceutical research Vol. 39; no. 8; pp. 1935 - 1944
• Main Authors: Wargniez, William, Connétable, Sophie, Bourokba, Nasrine, Dufour, Olivia, Nouveau, Stéphanie, Grégoire, Sébastien
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.08.2022; Springer; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Caffeine; Cheek; Medical Law; Menopause; Original Research Article; Pharmacology/Toxicology; Pharmacy; Post-menopause; Postmenopausal women; Skin; tape stripping; anatomical area; skin absorption; age
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-022-03311-0

Purpose Assessing the percutaneous absorption of cosmetic ingredients using in-vitro human skin reveals certain limitations, such as restricted anatomical sites and repeated exposure, and to overcome these issues, in-vivo studies are required. The aim of the study is to develop a robust non-invasive in-vivo protocol that should be applicable to a wide range of application. Methods A robust tape stripping protocol was therefore designed according to recent recommendations, and the impact of two different washing procedures on caffeine distribution in tape strips was investigated to optimise the protocol. The optimised protocol was then used to study the effect of age and anatomical area on the percutaneous absorption of caffeine, including facial areas which are not readily available for in-vitro studies. Results With tape stripping, a difference between the percutaneous absorption on the face (forehead, cheek) and the volar forearm was observed. No obvious difference was observed between percutaneous absorption in young and post-menopausal women, but this could be due to the limited number of subjects. Conclusion This tape stripping protocol is now to be deployed to address many other factors, such as percutaneous absorption in other anatomical areas (e.g. abdomen, axilla, etc.), impact of repeated applications and effect of formulation.