Genome size and base composition of five Pinus species from the Balkan region

• Published in: Plant cell reports Vol. 22; no. 1; pp. 59 - 62
• Main Authors: BOGUNIC, F, MURATOVIC, E, BROWN, S. C, SILJAK-YAKOVLEV, S
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.08.2003; Springer Nature B.V; Springer Verlag
• Subjects: Agronomy. Soil science and plant productions; Base Composition; Base Composition - genetics; Biological and medical sciences; Bosnia and Herzegovina; Bosnia-Herzegovina; Deoxyribonucleic acid; DNA; DNA, Plant; DNA, Plant - genetics; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genes. Genome; Genetic resources, diversity; Genetics and breeding of economic plants; Genome, Plant; Life Sciences; Molecular and cellular biology; Molecular genetics; Pine trees; Pinus; Pinus - genetics; Plant material; Triticum; Triticum - genetics; Vegetal Biology; Bosnia and Herzegovina; Pinus nigra; Flow cytometry; Ploidy; Interspecific and intraspecific variations; Balkan area; Dimension; Pinus; Pinus sylvestris; Softwood forest tree; Gymnospermae; Nucleotide; Coniferales; Spermatophyta; Chemical composition; Intraspecific comparison; Genome
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-003-0653-2

The 2C DNA content and base composition of five Pinus (2 n=24) species and two Pinus subspecies from the Balkan region have been estimated by flow cytometry. P. heldreichii (five populations) and P. peuce (one population) were assessed for the first time, as also were subspecies of P. nigra (three populations-two of subspecies nigra and one of subspecies dalmatica) along with P. sylvestris, and P. mugo from the same region. The 2C DNA values of these Pinus ranged from 42.5 pg to 54.9 pg (41.7-53.8 x 10(9)bp), and the base composition was quite stable (about 39.5% GC). Significant differences were observed between two subspecies of P. nigra and even between two populations of subsp. nigra. The two other species (P. sylvestris and P. mugo) had 2C values of 42.5 pg and 42.8 pg, respectively, while that of P. peuce was 54.9 pg. These genome sizes are in accordance with published values except for P. sylvestris, which was 20% below estimates made by other authors.