Identification of residues essential for carbohydrate recognition and cation dependence of the 46-kDa mannose 6-phosphate receptor

• Published in: Glycobiology (Oxford) Vol. 15; no. 11; pp. 1136 - 1149
• Main Authors: Sun, Guangjie, Zhao, Hongtao, Kalyanaraman, B, Dahms, Nancy M
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.11.2005; Oxford Publishing Limited (England)
• Subjects: 2-morpholinoethanesulfonic acid; Amino Acid Sequence; Animals; Asparagine - chemistry; Asparagine - metabolism; Aspartic Acid - chemistry; Aspartic Acid - metabolism; Carbohydrate Metabolism; cation-dependent mannose 6-phosphate receptor; cation-independent mannose 6-phosphate receptor; Cations - chemistry; Cattle; CD-MPR; CI-MPR; disuccinimidyl suberate; Dogs; DSS; EDTA; EGS; electron spin resonance; Electron Spin Resonance Spectroscopy; endo H; endo-β-N-acetylglucosaminidase H; ESR; ethylene diamine tetraacetic acid; ethylene glycol bis[succinimidylsuccinate]; Glucuronidase - metabolism; Histidine - chemistry; Histidine - metabolism; Humans; lectin; Lectins - chemistry; Lectins - physiology; lysosome; Lysosomes - enzymology; Lysosomes - metabolism; Man-6-P; Manganese - chemistry; mannose 6-phosphate receptor; mannose 6‐phosphate; MES; Mice; Molecular Sequence Data; MPR; protein targeting; Rats; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - physiology; SDS; sodium dodecyl sulphate; Surface Plasmon Resonance; Time Factors
• ISSN: 0959-6658; 1460-2423
• DOI: 10.1093/glycob/cwi098

The 46 kDa cation-dependent mannose 6-phosphate receptor (CD-MPR) plays an essential role in the biogenesis of lysosomes by diverting newly synthesized mannose 6-phosphate (Man-6-P)-containing lysosomal enzymes from the secretory pathway to acidified endosomes. Previous crystallographic studies of the CD-MPR have identified 11 amino acids within its carbohydrate binding pocket. These residues were evaluated quantitatively by assaying the binding affinity of mutant receptors containing a single amino acid substitution toward a lysosomal enzyme. The results show that substitution of Gln-66, Arg-111, Glu-133, or Tyr-143 results in a >800-fold decrease in affinity, demonstrating these four amino acids are essential for carbohydrate recognition by the CD-MPR. Solution binding and surface plasmon resonance analyses demonstrated that the presence of Mn²⁺ enhanced the affinity of the CD-MPR for a lysosomal enzyme by 2- to 4-fold and increased the stoichiometry of the interaction between a heterogeneous population of a lysosomal enzyme and the receptor by approximately 3-fold. In contrast, substitution of Asp-103 results in a protein that no longer exhibits enhanced binding affinities or altered stoichiometry in the presence of cations, and electron spin resonance demonstrated that the D103S mutant exhibits a 6-fold lower affinity for Mn²⁺ than the wild-type receptor (K[subscript d] = 3.7 6 1.4 mM versus 0.6 6 0.1 mM). Chemical cross-linking revealed that Mn²⁺ influences the stoichiometry of interaction between the CD-MPR and lysosomal enzymes by increasing the oligomeric state of the receptor from dimer to higher order oligomers. Taken together, these studies provide the molecular basis for high affinity carbohydrate recognition by the CD-MPR. Furthermore, Asp-103 has been identified as the key residue which mediates the effects of divalent cations on the binding properties of the CD-MPR.