Levansucrase optimization using solid state fermentation and levan biological activities studies

•Production of levansucrase from Bacillus subtilis by SSF technique.•A sequential optimization strategy for identifying the enzyme production parameters.•Antitumor and fibrinolytic activities for levan and quaternized levan were tested. Bacillus subtilis NRC1aza produced levansucrase under solid sta...

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Published inCarbohydrate polymers Vol. 96; no. 1; pp. 332 - 341
Main Authors Esawy, Mona A., Abdel-Fattah, Azza M., Ali, Mamdouh M., Helmy, Wafaa A., Salama, Bassem M., Taie, Hanan A.A., Hashem, Amal M., Awad, Ghada E.A.
Format Journal Article
LanguageEnglish
Published Kidlington Elsevier Ltd 01.07.2013
Elsevier
Subjects
Animals
Antineoplastic agents
Antineoplastic Agents - metabolism
Antineoplastic Agents - pharmacology
Antineoplastic Agents - therapeutic use
Antioxidants - metabolism
Antioxidants - pharmacology
Antioxidants - therapeutic use
Antitumor
Applied sciences
ascites
Bacillus subtilis
Bacillus subtilis - enzymology
bioactive properties
Biological and medical sciences
bioprocessing
Biotechnology
Carcinoma, Ehrlich Tumor - drug therapy
Chemotherapy
cisplatin
Enzyme engineering
Exact sciences and technology
experimental design
Female
Fermentation
Fibrinolytic agent
Fructans - pharmacology
Fructans - therapeutic use
Fundamental and applied biological sciences. Psychology
glucose
Hep G2 Cells
Hexosyltransferases - metabolism
Hexosyltransferases - pharmacology
Hexosyltransferases - therapeutic use
Humans
levan
Levansucrase
liver
MCF-7 Cells
Medical sciences
Methods. Procedures. Technologies
Mice
Natural polymers
neoplasm cells
Pharmacology. Drug treatments
Physicochemistry of polymers
Production of selected enzymes
Response surface methodology
Solid state fermentation
starch
Starch and polysaccharides
yeast extract
Response surface methodology
Levansucrase
Bacillus subtilis
Fibrinolytic agent
Solid state fermentation
Antitumor
Antineoplastic agent
Glycosyltransferases
Biosynthesis
Bacillaceae
Ehrlich ascite cell
Optimization
Bacillales
Bacteria
Tumor cell
Enzyme
Transferases
Experimental study
Fibrinolysis
In vitro
Biological activity
Operating conditions
Experimental design
Microorganism culture
Solid substrate fermentation
Oside polymer
Hexosyltransferases
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Abstract •Production of levansucrase from Bacillus subtilis by SSF technique.•A sequential optimization strategy for identifying the enzyme production parameters.•Antitumor and fibrinolytic activities for levan and quaternized levan were tested. Bacillus subtilis NRC1aza produced levansucrase under solid state fermentation using starch as support. A sequential optimization strategy, based on statistical experimental designs is employed to enhance enzyme productivity. First, a 2-level Plackett–Burman design was applied for bioprocess parameters screen that significantly increase levansucrase production. Second optimization step was performed using fractional factorial design in order to optimize the amounts of highest positive variables that had significant effect on levansucrase productivity. Maximal enzyme productivity of 170U/gds was achieved in presence of glucose, yeast extract, and pH 8. In vitro, experiments confirmed that LevCR and LevQT had an antitumor activity against different animal and human cancer cell lines by demonstrating inhibitory effects on growth of Ehrlich ascites carcinoma cell line, human MCF-7 breast and liver HepG2 cancer cell lines, in particular LevQT was found to be efficacious compared to anticancer drug, cisplatin. Result focused in LevCR as strong fibrinolytic agent.
AbstractList Bacillus subtilis NRC1aza produced levansucrase under solid state fermentation using starch as support. A sequential optimization strategy, based on statistical experimental designs is employed to enhance enzyme productivity. First, a 2-level Plackett–Burman design was applied for bioprocess parameters screen that significantly increase levansucrase production. Second optimization step was performed using fractional factorial design in order to optimize the amounts of highest positive variables that had significant effect on levansucrase productivity. Maximal enzyme productivity of 170U/gds was achieved in presence of glucose, yeast extract, and pH 8. In vitro, experiments confirmed that LevCR and LevQT had an antitumor activity against different animal and human cancer cell lines by demonstrating inhibitory effects on growth of Ehrlich ascites carcinoma cell line, human MCF-7 breast and liver HepG2 cancer cell lines, in particular LevQT was found to be efficacious compared to anticancer drug, cisplatin. Result focused in LevCR as strong fibrinolytic agent.
Bacillus subtilis NRC1aza produced levansucrase under solid state fermentation using starch as support. A sequential optimization strategy, based on statistical experimental designs is employed to enhance enzyme productivity. First, a 2-level Plackett-Burman design was applied for bioprocess parameters screen that significantly increase levansucrase production. Second optimization step was performed using fractional factorial design in order to optimize the amounts of highest positive variables that had significant effect on levansucrase productivity. Maximal enzyme productivity of 170 U/gds was achieved in presence of glucose, yeast extract, and pH 8. In vitro, experiments confirmed that LevCR and LevQT had an antitumor activity against different animal and human cancer cell lines by demonstrating inhibitory effects on growth of Ehrlich ascites carcinoma cell line, human MCF-7 breast and liver HepG2 cancer cell lines, in particular LevQT was found to be efficacious compared to anticancer drug, cisplatin. Result focused in LevCR as strong fibrinolytic agent.
•Production of levansucrase from Bacillus subtilis by SSF technique.•A sequential optimization strategy for identifying the enzyme production parameters.•Antitumor and fibrinolytic activities for levan and quaternized levan were tested. Bacillus subtilis NRC1aza produced levansucrase under solid state fermentation using starch as support. A sequential optimization strategy, based on statistical experimental designs is employed to enhance enzyme productivity. First, a 2-level Plackett–Burman design was applied for bioprocess parameters screen that significantly increase levansucrase production. Second optimization step was performed using fractional factorial design in order to optimize the amounts of highest positive variables that had significant effect on levansucrase productivity. Maximal enzyme productivity of 170U/gds was achieved in presence of glucose, yeast extract, and pH 8. In vitro, experiments confirmed that LevCR and LevQT had an antitumor activity against different animal and human cancer cell lines by demonstrating inhibitory effects on growth of Ehrlich ascites carcinoma cell line, human MCF-7 breast and liver HepG2 cancer cell lines, in particular LevQT was found to be efficacious compared to anticancer drug, cisplatin. Result focused in LevCR as strong fibrinolytic agent.
Author Abdel-Fattah, Azza M.
Awad, Ghada E.A.
Taie, Hanan A.A.
Salama, Bassem M.
Esawy, Mona A.
Helmy, Wafaa A.
Hashem, Amal M.
Ali, Mamdouh M.
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  givenname: Azza M.
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  givenname: Wafaa A.
  surname: Helmy
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  givenname: Bassem M.
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  givenname: Hanan A.A.
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  givenname: Amal M.
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  givenname: Ghada E.A.
  surname: Awad
  fullname: Awad, Ghada E.A.
  email: ghadaawad18@yahoo.com, ghadaawad18@gmail.com
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Issue 1
Keywords Response surface methodology
Levansucrase
Bacillus subtilis
Fibrinolytic agent
Solid state fermentation
Antitumor
Antineoplastic agent
Glycosyltransferases
Biosynthesis
Bacillaceae
Ehrlich ascite cell
Optimization
Bacillales
Bacteria
Tumor cell
Enzyme
Transferases
Experimental study
Fibrinolysis
In vitro
Biological activity
Operating conditions
Experimental design
Microorganism culture
Solid substrate fermentation
Oside polymer
Hexosyltransferases
Language English
License CC BY 4.0
Copyright © 2013 Elsevier Ltd. All rights reserved.
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Snippet •Production of levansucrase from Bacillus subtilis by SSF technique.•A sequential optimization strategy for identifying the enzyme production...
Bacillus subtilis NRC1aza produced levansucrase under solid state fermentation using starch as support. A sequential optimization strategy, based on...
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SubjectTerms Animals
Antineoplastic agents
Antineoplastic Agents - metabolism
Antineoplastic Agents - pharmacology
Antineoplastic Agents - therapeutic use
Antioxidants - metabolism
Antioxidants - pharmacology
Antioxidants - therapeutic use
Antitumor
Applied sciences
ascites
Bacillus subtilis
Bacillus subtilis - enzymology
bioactive properties
Biological and medical sciences
bioprocessing
Biotechnology
Carcinoma, Ehrlich Tumor - drug therapy
Chemotherapy
cisplatin
Enzyme engineering
Exact sciences and technology
experimental design
Female
Fermentation
Fibrinolytic agent
Fructans - pharmacology
Fructans - therapeutic use
Fundamental and applied biological sciences. Psychology
glucose
Hep G2 Cells
Hexosyltransferases - metabolism
Hexosyltransferases - pharmacology
Hexosyltransferases - therapeutic use
Humans
levan
Levansucrase
liver
MCF-7 Cells
Medical sciences
Methods. Procedures. Technologies
Mice
Natural polymers
neoplasm cells
Pharmacology. Drug treatments
Physicochemistry of polymers
Production of selected enzymes
Response surface methodology
Solid state fermentation
starch
Starch and polysaccharides
yeast extract
Title Levansucrase optimization using solid state fermentation and levan biological activities studies
URI https://dx.doi.org/10.1016/j.carbpol.2013.03.089
https://www.ncbi.nlm.nih.gov/pubmed/23688489
https://search.proquest.com/docview/1353984881
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