Levansucrase optimization using solid state fermentation and levan biological activities studies

• Published in: Carbohydrate polymers Vol. 96; no. 1; pp. 332 - 341
• Main Authors: Esawy, Mona A., Abdel-Fattah, Azza M., Ali, Mamdouh M., Helmy, Wafaa A., Salama, Bassem M., Taie, Hanan A.A., Hashem, Amal M., Awad, Ghada E.A.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2013; Elsevier
• Subjects: Animals; Antineoplastic agents; Antineoplastic Agents - metabolism; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Antioxidants - metabolism; Antioxidants - pharmacology; Antioxidants - therapeutic use; Antitumor; Applied sciences; ascites; Bacillus subtilis; Bacillus subtilis - enzymology; bioactive properties; Biological and medical sciences; bioprocessing; Biotechnology; Carcinoma, Ehrlich Tumor - drug therapy; Chemotherapy; cisplatin; Enzyme engineering; Exact sciences and technology; experimental design; Female; Fermentation; Fibrinolytic agent; Fructans - pharmacology; Fructans - therapeutic use; Fundamental and applied biological sciences. Psychology; glucose; Hep G2 Cells; Hexosyltransferases - metabolism; Hexosyltransferases - pharmacology; Hexosyltransferases - therapeutic use; Humans; levan; Levansucrase; liver; MCF-7 Cells; Medical sciences; Methods. Procedures. Technologies; Mice; Natural polymers; neoplasm cells; Pharmacology. Drug treatments; Physicochemistry of polymers; Production of selected enzymes; Response surface methodology; Solid state fermentation; starch; Starch and polysaccharides; yeast extract; Response surface methodology; Levansucrase; Bacillus subtilis; Fibrinolytic agent; Solid state fermentation; Antitumor; Antineoplastic agent; Glycosyltransferases; Biosynthesis; Bacillaceae; Ehrlich ascite cell; Optimization; Bacillales; Bacteria; Tumor cell; Enzyme; Transferases; Experimental study; Fibrinolysis; In vitro; Biological activity; Operating conditions; Experimental design; Microorganism culture; Solid substrate fermentation; Oside polymer; Hexosyltransferases
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.03.089

•Production of levansucrase from Bacillus subtilis by SSF technique.•A sequential optimization strategy for identifying the enzyme production parameters.•Antitumor and fibrinolytic activities for levan and quaternized levan were tested. Bacillus subtilis NRC1aza produced levansucrase under solid state fermentation using starch as support. A sequential optimization strategy, based on statistical experimental designs is employed to enhance enzyme productivity. First, a 2-level Plackett–Burman design was applied for bioprocess parameters screen that significantly increase levansucrase production. Second optimization step was performed using fractional factorial design in order to optimize the amounts of highest positive variables that had significant effect on levansucrase productivity. Maximal enzyme productivity of 170U/gds was achieved in presence of glucose, yeast extract, and pH 8. In vitro, experiments confirmed that LevCR and LevQT had an antitumor activity against different animal and human cancer cell lines by demonstrating inhibitory effects on growth of Ehrlich ascites carcinoma cell line, human MCF-7 breast and liver HepG2 cancer cell lines, in particular LevQT was found to be efficacious compared to anticancer drug, cisplatin. Result focused in LevCR as strong fibrinolytic agent.