Screening and application of a broad-spectrum aptamer for acyclic guanosine analogues

• Published in: Analytical and bioanalytical chemistry Vol. 413; no. 19; pp. 4855 - 4863
• Main Authors: Ren, Le, Qi, Shuo, Khan, Imran Mahmood, Wu, Shijia, Duan, Nuo, Wang, Zhouping
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2021; Springer; Springer Nature B.V
• Subjects: Acyclovir; Analysis; Analytical Chemistry; Antiviral agents; Aptamers; Biochemistry; Biotechnology; Capillary electrophoresis; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chronic toxicity; Cytomegalovirus; Disease resistance; Drugs; Famciclovir; Fluorescence; Food Science; Ganciclovir; Graphene; Guanosine; Guanosines; Identification and classification; Laboratories; Laboratory Medicine; Monitoring/Environmental Analysis; Next-generation sequencing; Oligonucleotides; Penciclovir; Polymerase chain reaction; Properties; Research Paper; Spectra; Toxicity; Veterinary medicine; China; Capture-SELEX; Fluorescence; Acyclic guanosine analogues; Polarization; Aptamer
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-021-03446-w

Acyclic guanosine analogues, a class of widely used antiviral drugs, can cause chronic toxicity and virus resistance. Therefore, it is essential to establish rapid and accurate methods to detect acyclic guanosine analogues. In this study, five acyclic guanosine analogues (acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir) were used as positive targets to obtain broad-spectrum aptamers through Capture-SELEX technology. Real-time quantitative PCR (Q-PCR) was used to monitor the aptamer SELEX process. After the sixteen rounds of selection against mixed targets, sequences were obtained by high-throughput sequencing (HTS). Furthermore, a broad-spectrum aptamer, named CIV6, was found as the higher performance aptamer that was suitable for five acyclic guanosine analogues by graphene oxide (GO) polarization and fluorescence assay. Finally, the aptamer CIV6 was used to construct GO fluorescence assay to detect five acyclic guanosine analogues. The limits of detection (LOD) of acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir were 0.48 ng·mL −1 , 0.53 ng·mL −1 , 0.50 ng·mL −1 , 0.56 ng·mL −1 , and 0.38 ng·mL −1 , respectively. Graphical abstract