Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map

• Published in: Theoretical and applied genetics Vol. 102; no. 2-3; pp. 206 - 214
• Main Authors: SANKAR, A. A, MOORE, G. A
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.02.2001; Berlin Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Citrus; Citrus fruits; Classical and quantitative genetics. Population genetics. Molecular genetics; Cultivars; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetics and breeding of economic plants; Genomes; Horticulture; Laboratories; Population genetics; Genetic mapping; Linkage; Genetic marker; Citrus fruit; Rutaceae; Citrus grandis; Poncirus trifoliata; Polymerase chain reaction; Dicotyledones; Microsatellite DNA; Angiospermae; Spermatophyta; Polymorphism
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051637

Inter-simple sequence repeat (ISSR) analysis was evaluated for its usefulness in generating markers to extend the genetic linkage map of Citrus using a backcross population previously mapped with restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and isozyme markers. ISSR markers were obtained through the simple technique of PCR followed by analysis on agarose gels, using simple sequence repeat (SSR) primers. Optimization of reaction conditions was achieved for 50% of the SSR primers screened, and the primers amplified reproducible polymorphic bands in the parents and progeny of the backcross population. Mendelian segregation of the polymorphic bands was demonstrated, with an insignificant number of skewed loci. Most of the SSR primers produced dominant loci; however co-dominance was observed with loci derived from three primers. A new genetic map was produced by combining the segregation data for the ISSR markers and data for the RFLP, RAPD and isozyme markers from the previous map and creating genetic linkages among all the markers using JoinMap 2.0 mapping software. The new map has an improved distribution of markers along the linkage groups with fewer gaps, and marker order showed partial or complete conservation in the linkage groups. The incorporation of ISSR markers into the genetic linkage map demonstrates that ISSR markers are suitable for genetic mapping in Citrus.