Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia

• Published in: Parasitology research (1987) Vol. 119; no. 9; pp. 2983 - 2990
• Main Authors: Wardhana, April Hari, Sawitri, Dyah Haryuningtyas, Ekawasti, Fitrine, Martindah, Eny, Apritadewi, Dias, Shibahara, Tomoyuki, Kusumoto, Masahiro, Tokoro, Masaharu, Sasai, Kazumi, Matsubayashi, Makoto
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2020; Springer; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Entamoeba; Entamoeba polecki; Epidemiology; Health aspects; Hogs; Immunology; Infection; Medical Microbiology; Microbiology; Prevalence studies (Epidemiology); Protozoology - Original Paper; Species; Statistical analysis; Swine; Virulence; Virulence (Microbiology); Japan; Indonesia; West Java Indonesia; Gastrointestinal parasites; Subtype; Indonesia
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-020-06806-0

Entamoeba suis and E . polecki subtype (ST) 1 and ST3 recently have been inferred to be virulent in pigs. However, because relevant molecular epidemiological surveys have been limited, the prevalences of these species remain unknown and their pathogenicities are still controversial. We surveyed 196 fecal samples of pigs (118 of adults, 78 of piglets) at Tangerang in West Java, Indonesia, in 2017, employing PCR using porcine Entamoeba -specific primers. E . suis was the more frequently detected species, observed in 81.1% of samples, while E . polecki ST1 and ST3 were detected in 18.4% and 17.3% of samples, respectively; mixed infections (harboring 2–3 species or subtypes of Entamoeba ) were confirmed in 29.3% of positive samples. Statistically significant differences in the positive rates were not seen between adult pigs and piglets, except for those of E . polecki ST3. The prevalences of Eimeria spp. and/or Cystoisospora suis (79.1%), strongyles (55.6%), and Strongyloides spp. (6.1%) were also observed morphologically in the samples. Further chronological or seasonal investigations of pigs and humans in these high-prevalence areas are needed to assess the virulence of the Entamoeba parasites, including the effects on pig productivity, and to evaluate the zoonotic impacts of these organisms.