In vitro study of the hemocompatibility of superparamagnetic contrast agent for magnetic resonance imaging

• Published in: Clinical materials Vol. 15; no. 4; pp. 293 - 301
• Main Authors: Chouly, C., Bordenave, L., Bareille, R., Guerin, V., Baquey, A., Pouliquen, D., Baquey, C.H., Jallet, P.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 1994; Oxford Elsevier
• Subjects: Animals; Biological and medical sciences; Blood Coagulation; Cattle; Complement Activation; Contrast Media - chemistry; Contrast Media - toxicity; Contrast media. Radiopharmaceuticals; Cytotoxicity, Immunologic; Hemolysis; Histocompatibility; Iron-Dextran Complex - toxicity; Magnetic Resonance Imaging; Materials Testing; Medical sciences; Mice; Particle Size; Pharmacology. Drug treatments; Platelet Aggregation; Human; Blood coagulation; Cell culture; Endothelial cell; Rodentia; Paramagnetic contrast agent; Cytotoxicity; Nuclear magnetic resonance imaging; Blood; Dextran; Vertebrata; Blood cell; Mammalia; Magnetite; Mouse; Animal; Platelet function; Biocompatibility
• ISSN: 0267-6605; 1878-6979
• DOI: 10.1016/0267-6605(94)90061-2

Five different nanoparticles, potentially useful in magnetic resonance imaging (MRI) after venous administration, were studied for their hemo-compatibility. The in vitro methodology evaluated these materials by several parameters: cytotoxicity towards cells cultured in vitro, aggregation ability of platelets, hemolysis inducibility, intrinsic and extrinsic coagulation pathway activation, and complement activation. With the proposed clinical dose, regardless of the cell type used (murine cell line or human endothelial cells) no toxicity was observed. The presence of the particles in blood did not produce any considerable damage: either hemolysis or platelet aggregation or blood coagulation were recorded. However, a slight decrease in aggregation ability of platelets was noticed as well as an increase in partial thromboplastin time. Because of the quick removal of the particles from the bloodstream, these phenomena must be short-lived, thus avoiding significant adverse clinical effects.