Expression of selected cytochrome P450 isoforms and of cooperating enzymes in colorectal tissues in selected pathological conditions

The current interest in CYP expression in the colon results from its uniqueness as a target organ for cancer. To date, the CYP expression profiles in the colon have not yet been subject of comprehensive research. In this study, we investigated 40 patients with Crohn's disease, 40 with ulcerativ...

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Published inPathology, research and practice Vol. 210; no. 4; pp. 242 - 249
Main Authors Plewka, Danuta, Plewka, Andrzej, Szczepanik, Tomasz, Morek, Michał, Bogunia, Edyta, Wittek, Piotr, Kijonka, Czarosław
Format Journal Article
LanguageEnglish
Published Germany Elsevier GmbH 01.04.2014
Subjects
Adult
Colitis, Ulcerative - enzymology
Colorectal tissues
Crohn Disease - enzymology
Crohn's disease
Cytochrome P-450 Enzyme System - analysis
Cytochrome P-450 Enzyme System - biosynthesis
Cytochrome P450
Female
Humans
Immunohistochemistry
Isoenzymes - analysis
Isoenzymes - biosynthesis
Male
Middle Aged
Transcriptome
Ulcerative colitis
Colorectal tissues
Crohn's disease
Cytochrome P450
Ulcerative colitis
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Summary:The current interest in CYP expression in the colon results from its uniqueness as a target organ for cancer. To date, the CYP expression profiles in the colon have not yet been subject of comprehensive research. In this study, we investigated 40 patients with Crohn's disease, 40 with ulcerative colitis, and 40 healthy subjects as a control group. Colon tissues were fixed, dehydrated, cleared in xylene and embedded in paraffin. Sections were prepared from paraffin blocks for immunohistochemical staining with specific antibodies. We used antibodies to the human CYP1A1, CYP2B6, CYP2C9, CYP2E1 and CYP3A4 isoforms, as well as antibodies to the human glycoprotein P, glutathione-S transferase and antibody to the UDP-glucuronosyltransferase. The sections were stained immunohistochemically and examined using light microscopy. Cellular localization was determined, and computer image analysis was used. In all cases with Crohn's disease, the proteins studied showed at least a twofold expression. Ulcerative colitis showed a much weaker influence regarding the expression of the proteins studied but in case of CYP2C9 and UDP-glucuronosyltransferase, a decrease of expression was observed.
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ISSN:0344-0338
1618-0631
1618-0631
DOI:10.1016/j.prp.2013.12.010