Whole genome analysis of a novel Spodoptera exigua nucleopolyhedrovirus isolate (SeMNPV-IR) to Iran

• Published in: Biológia Vol. 78; no. 9; pp. 2563 - 2574
• Main Authors: Eroglu, Gozde Busra, Karimi, Javad
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.09.2023; Springer Nature B.V
• Subjects: Agricultural production; Biological control; Biomedical and Life Sciences; Cell Biology; Crop damage; Crop production; Economic impact; Endonuclease; Genomes; Homology; Larvae; Life Sciences; Microbiology; Microorganism control agents; Microorganisms; Open reading frames; Original Article; Pest control; Pests; Phylogeny; Plant Sciences; Spodoptera exigua; Zoology; Iran; Multiple nucleopolyhedroviruses; Geographic differences; Full genome analysis; Baculoviral genomics; Spodoptera exigua
• ISSN: 1336-9563; 0006-3088; 1336-9563
• DOI: 10.1007/s11756-023-01399-2

Baculoviruses are successful microbial control agents used in the biological control of agricultural pest species, especially in the order Lepidoptera. The beet armyworm, Spodoptera exigua is a popular agricultural pest in the world. S exigua larvae, which are active in the all-summer period, cause economic losses by damaging many crops in agricultural production areas. This article aims to analyze the full genome of Spodoptera exigua multiple nucleopolyhedroviruses from Iran (SeMNPV-IR) and to determine the geographical difference between the strains at the genomic level. The full genome of SeMNPV-IR is 135.764 base pairs in length that contained 136 open reading frames (ORFs), and 43.92% G + C content. The seven homologous repeated ( hr ) regions were identified. In the results of genome-wide phylogenetic analysis, it was determined that the SeMNPV-IR genome isolated from Iran was interestingly close to the genome of the US and Korea isolates. However, there are significant differences in the two hypothetical ( Orf 83 and Orf 104) genes. The SeMNPV-IR has a unique homolog repeat region (hr1, 96 bp) that is not found in other SeMNPV genomes, and it also differs in terms of the hr2 region. In silico restriction endonuclease analysis by Stu I and Sac II enzymes show that there were significant differences between all geographic isolates of SeMNPV.