Box–Behnken design based statistical modeling for ultrasound-assisted extraction of corn silk polysaccharide

• Published in: Carbohydrate polymers Vol. 92; no. 1; pp. 604 - 611
• Main Authors: Prakash Maran, J., Manikandan, S., Thirugnanasambandham, K., Vigna Nivetha, C., Dinesh, R.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 30.01.2013; Elsevier
• Subjects: Applied sciences; Biological and medical sciences; Box–Behnken design; Cereal and baking product industries; Chromatography, High Pressure Liquid; Corn silk; equations; Exact sciences and technology; Extraction; Food industries; Fundamental and applied biological sciences. Psychology; Models, Statistical; Natural polymers; Physicochemistry of polymers; Polysaccharide; polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Regression Analysis; Silk - chemistry; Sound; Starch and polysaccharides; statistical models; styles (flowers); temperature; Ultrasonics; Ultrasound; Zea mays - chemistry; Corn silk; Extraction; Box–Behnken design; Ultrasound; Polysaccharide; Response surface methodology; Experimental study; Solvent extraction; Optimization; Operating conditions; Experimental design; Plant origin; Oside polymer; Corn by product; Ultrasound assisted process; Pistil; Box-Behnken design
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.09.020

► UAE of polysaccharide from corn silk. ► Box–Behnken design was applied to find out the optimum conditions. ► Second order polynomial regression models were developed. ► Optimal conditions were determined by Derringer's desired function methodology. ► Experimental values showed good agreement with the values predicted by the model. In this study, ultrasound assisted extraction (UAE) conditions on the yield of polysaccharide from corn silk were studied using three factors, three level Box–Behnken response surface design. Process parameters, which affect the efficiency of UAE such as extraction temperature (40–60°C), time (10–30min) and solid–liquid ratio (1:10–1:30g/ml) were investigated. The results showed that, the extraction conditions have significant effects on extraction yield of polysaccharide. The obtained experimental data were fitted to a second-order polynomial equation using multiple regression analysis with high coefficient of determination value (R2) of 0.994. An optimization study using Derringer's desired function methodology was performed and the optimal conditions based on both individual and combinations of all independent variables (extraction temperature of 56°C, time of 17min and solid–liquid ratio of 1:20g/ml) were determined with maximum polysaccharide yield of 6.06%, which was confirmed through validation experiments.