Analysis of Lupinus albus heat-shock granule proteins in response to high temperature stress

• Published in: Biologia plantarum Vol. 54; no. 3; pp. 587 - 591
• Main Authors: Cherian, S.,Universidade Nova de Lisboa (Portugal). Inst. de Tecnologia Quimica e Biologica, Ferreira, R.B.,Universidade Nova de Lisboa (Portugal). Inst. de Tecnologia Quimica e Biologica
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2010; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: Biomedical and Life Sciences; Brief Communication; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; ESTRES TERMICO; GRANULE; GRANULES; GRANULOS; HEAT SHOCK PROTEINS; HEAT STRESS; http://www.fao.org/aos/agrovoc#c_11488; http://www.fao.org/aos/agrovoc#c_2523; http://www.fao.org/aos/agrovoc#c_3357; http://www.fao.org/aos/agrovoc#c_36493; http://www.fao.org/aos/agrovoc#c_4464; http://www.fao.org/aos/agrovoc#c_4810; http://www.fao.org/aos/agrovoc#c_8045; Life Sciences; LUPINUS ALBUS; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; molecular chaperone; Plant Sciences; PROTEINAS DE SHOCK TERMICO; PROTEINE DE CHOC THERMIQUE; small heat-shock proteins; STRESS THERMIQUE; thermotolerance; ULTAESTRUCTURA; ULTRASTRUCTURE; thermotolerance; molecular chaperone; small heat-shock proteins
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-010-0106-z

An important aspect of heat-shock response of lupin (Lupinus albus cv. Rio Maior) is the formation of cytoplasmic granular aggregates, called heat-shock granules (HSGs). In this study, two-dimensional electrophoresis (2-DE) was used to detect the component proteins of HSG complexes formed in vivo. Evaluation of 2-DE revealed differential expression of several proteins under heat shock conditions when compared with control. Among them, small heat-shock proteins (sHSPs) of 15 to 30 kDa were found to be the major representative proteins along with other proteins of relative molecular mass ranging from 36 to 45 kDa and above.