Ex vitro acclimatization of plantain plantlets micropropagated in temporary immersion bioreactor

• Published in: Biologia plantarum Vol. 54; no. 2; pp. 237 - 244
• Main Authors: Aragon, C.,Universidade Tecnica de Lisboa (Portugal). Departamento de Botanica e Engenharia Biologia, Carvalho, L.,Universidade Tecnica de Lisboa (Portugal). Departamento de Botanica e Engenharia Biologia, Gonzalez, J.,Universidad de Ciego de Avila (Cuba). Laboratorio de Celulas y Cultivo Tejidos, Escalona, M.,Universidad de Ciego de Avila (Cuba). Laboratorio de Celulas y Cultivo Tejidos, Amancio, S.,Universidade Tecnica de Lisboa (Portugal). Departamento de Botanica e Engenharia Biologia
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2010; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: ACIDE ASCORBIQUE; ACIDO ASCORBICO; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ADAPTACION; ADAPTATION; ascorbate-glutathione cycle; ASCORBIC ACID; BANANE PLANTAIN; Biomedical and Life Sciences; BIOREACTEUR; BIOREACTORS; BIORREACTORES; CATALASA; CATALASE; CELLS; CELLULE; CELULAS; CRECIMIENTO; CROISSANCE; CULTIVO IN VITRO; CULTURE IN VITRO; CULTURE MEDIA; ENZYME ACTIVITY; ESTOMA; ESTRES; ex vitro growth; EXPRESION GENICA; EXPRESSION DES GENES; FREE RADICALS; GENE EXPRESSION; GLUTACION; GLUTATHION; GLUTATHIONE; GROWTH; http://www.fao.org/aos/agrovoc#c_10204; http://www.fao.org/aos/agrovoc#c_11184; http://www.fao.org/aos/agrovoc#c_117; http://www.fao.org/aos/agrovoc#c_13251; http://www.fao.org/aos/agrovoc#c_1380; http://www.fao.org/aos/agrovoc#c_1418; http://www.fao.org/aos/agrovoc#c_24091; http://www.fao.org/aos/agrovoc#c_24135; http://www.fao.org/aos/agrovoc#c_24136; http://www.fao.org/aos/agrovoc#c_2604; http://www.fao.org/aos/agrovoc#c_27469; http://www.fao.org/aos/agrovoc#c_27527; http://www.fao.org/aos/agrovoc#c_32817; http://www.fao.org/aos/agrovoc#c_33488; http://www.fao.org/aos/agrovoc#c_3394; http://www.fao.org/aos/agrovoc#c_34114; http://www.fao.org/aos/agrovoc#c_4993; http://www.fao.org/aos/agrovoc#c_5472; http://www.fao.org/aos/agrovoc#c_5474; http://www.fao.org/aos/agrovoc#c_5989; http://www.fao.org/aos/agrovoc#c_661; http://www.fao.org/aos/agrovoc#c_6945; http://www.fao.org/aos/agrovoc#c_7423; http://www.fao.org/aos/agrovoc#c_7452; http://www.fao.org/aos/agrovoc#c_7865; IMMUNOBLOTTING; IN VITRO CULTURE; IN VITRO REGENERATION; Life Sciences; MEDIO DE CULTIVO; MICROPROPAGACION; MICROPROPAGATION; MILIEU DE CULTURE; MUSA; Original Papers; OXIDACION; OXIDATION; oxidative stress; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDATION; OXYDOREDUCTASE; PEROXIDASAS; PEROXIDASES; PEROXYDASE; Plant Sciences; PLANTAINS; PLANTULAS; PLANTULE; PLATANO; RADICAL LIBRE; RADICALES LIBRES; REGENERACION IN VITRO; REGENERATION IN VITRO; SEEDLINGS; STOMATA; STOMATE; STRESS; SUPEROXIDE DISMUTASE; SUPEROXIDO DISMUTASA; SUPEROXYDE DISMUTASE; tib culture; TRANSFERASE; TRANSFERASES; TRANSFERIAS; growth; ascorbate-glutathione cycle; TIB culture; oxidative stress
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-010-0042-y

Plantain (Musa ABB CEMSA 3/4) plantlets were micropropagated in temporary immersion bioreactors (TIB) or in gelled medium (GM). After ex vitro transfer ROS accumulation was determined by infiltrating leaves with nitroblue tetrazolium (NBT) and 3,3'-diaminobenzidine (DAB). Stomatal cells were more stained with NBT and DAB in GM plants than in TIB plants, but the difference disappeared at the end of acclimatization. At the end of the in vitro phase, GM plantlets showed higher activities of ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase (MDHAR) and glutathione reductase, while activities of catalase (CAT), superoxide dismutase (SOD) and glutathione transferase (GT) were higher in TIB grown plantlets. At the end of acclimatization GT, SOD, CAT and MDHAR stabilized at low values of activity in plantlets derived from both treatments. Concerning the correspondent genes, GM plantlets showed higher expression of all transcripts with the exception of CuZnSOD. The immunobloting of peroxiredoxins (PRXs) showed that chloroplast-located PRXs were expressed at higher levels in TIB plantlets, some showing polymerization. In conclusion, TIB grown plantlets had an improved anti-oxidative response when compared with GM.