Affinibrenneria salicis gen. nov. sp. nov. isolated from Salix matsudana bark canker

• Published in: Archives of microbiology Vol. 203; no. 6; pp. 3473 - 3481
• Main Authors: Bian, Dan-ran, Xue, Han, Wang, Guang-ming, Piao, Chun-gen, Li, Yong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2021; Springer Nature B.V
• Subjects: Amino acids; Bark; Biochemistry; Biomedical and Life Sciences; Biotechnology; Canker; Cell Biology; Ecology; Fatty acids; Genes; Genomes; Life Sciences; Microbial Ecology; Microbiology; New species; Nucleotides; Original Paper; Pathogens; Phylogeny; RpoB protein; rRNA 16S; Salix matsudana; Virulence; Willow; Average nucleotide identity; Phylogenomics; Multilocus sequence analysis
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02323-5

L3-3HA T , a Gram-negative-staining, facultatively anaerobic, motile bacterial strain, was isolated from the symptomatic bark of Salix matsudana canker in China. 16S rRNA gene analysis revealed that the novel strain shares the highest sequence similarity with Brenneria goodwinii FRB141 T (95.5%). In phylogenetic trees based on four housekeeping genes ( gyr B, rpo B, atp D, and inf B) and the 16S rRNA gene sequence, the novel strain formed a separate branch from the five genera of the family Pectobacteriaceae ( Lonsdalea , Brenneria , Dickeya , Pectobacterium , and Sodalis ), suggesting that the novel strain should belong to a novel species of a novel genus within the family Pectobacteriaceae . The result was also supported by phylogenomics, amino acid identity and average nucleotide identity. The major fatty acids were C 14:0 , C 16:0 , C 17:0 cyclo, and C 19:0 cyclo ɷ 8 c . Genome analysis showed that the novel strain has a large genome (5.89 Mb) with 5,052 coding genes, including 181 virulence genes by searching the pathogen–host interactions database (PHI-base), indicating that the novel strain is a potential pathogen of plants and animals. Based on phenotypic and genotypic characteristics, the L3-3HA T strain represents a novel species of a novel genus in the Pectobacteriaceae family, for which the name Affinibrenneria salicis gen nov. sp. nov. is proposed. The strain type is L3-3HA T (= CFCC 15588 T  = LMG 31209 T ).