Possible Roles of Carbohydrate Management and Cytokinin in the Process of Defoliation-Regrowth Cycles in Rice

• Published in: International journal of molecular sciences Vol. 25; no. 10; p. 5070
• Main Authors: Sakashita, Yuki, Kurashima, Hikaru, Fukuda, Mika, Hirano, Haru, Lamsal, Sagar, Katayama, Naoki, Fukao, Takeshi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.05.2024
• Subjects: Analysis; Carbohydrate Metabolism; Carbohydrates; Chlorophyll; Chlorophyll - metabolism; cytokinin; Cytokinins; Cytokinins - metabolism; Enzymes; Gene Expression Regulation, Plant; Genes; Genomes; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; Grasses; Leaves; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Oryza sativa; Photosynthesis; Photosystem II Protein Complex - metabolism; Physiological aspects; Physiology; Plant Leaves - genetics; Plant Leaves - growth & development; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Production processes; regrowth vigor; RNA; Sucrose; sucrose synthase; Japan; regrowth vigor; Oryza sativa; cytokinin; photosynthesis; sucrose synthase
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25105070

Defoliation is an inevitable abiotic stress for forage and turf grasses because harvesting, grazing, and mowing are general processes for their production and management. Vegetative regrowth occurs upon defoliation, a crucial trait determining the productivity and persistence of these grasses. However, the information about the molecular regulation of this trait is limited because it is still challenging to perform molecular analyses in forage and turf grasses. Here, we used rice as a model to investigate vegetative regrowth upon defoliation at physiological and molecular levels. This study analyzed stubble and regrown leaves following periodic defoliation using two rice varieties with contrasting regrowth vigor. Vigorous regrowth was associated with maintained chlorophyll content and photosystem II performance; a restricted and promoted mRNA accumulation of sucrose synthase (SUS) I and III subfamilies, respectively; and reduced enzymatic activity of SUS. These results suggest that critical factors affecting vegetative regrowth upon defoliation are de novo carbohydrate synthesis by newly emerged leaves and proper carbohydrate management in leaves and stubble. Physiological and genetic analyses have demonstrated that the reduced sensitivity to and inhibited biosynthesis of cytokinin enhance regrowth vigor. Proper regulation of these metabolic and hormonal pathways identified in this study can lead to the development of new grass varieties with enhanced regrowth vigor following defoliation.