Characteristics of bioemulsifiers synthesised in crude oil media by Halomonas eurihalina and their effectiveness in the isolation of bacteria able to grow in the presence of hydrocarbons

Halomonas eurihalina strains F2-7, H28, H96, H212 and H214 were capable of producing large amounts of exopolysaccharides (EPS) in MY medium with added crude oil. The biopolymers showed lower carbohydrate and protein content than those synthesised in control medium without oil. Nevertheless, the perc...

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Published inApplied microbiology and biotechnology Vol. 60; no. 3; pp. 347 - 351
Main Authors CALVO, C, MARTINEZ-CHECA, F, TOLEDO, F. L, PORCEL, J, QUESADA, E
Format Journal Article
LanguageEnglish
Published Berlin Springer 01.11.2002
Springer Nature B.V
Subjects
Analysis
Bacillus
Bacteria - growth & development
Bacteria - isolation & purification
Biodegradation, Environmental
Biological and medical sciences
Biopolymers
Biosynthesis
Crude oil
Excipients - metabolism
Excipients - pharmacology
exopolysaccharides
Fundamental and applied biological sciences. Psychology
Halomonas
Halomonas - metabolism
Halomonas eurihalina
Hydrocarbons
Molecular microbiology
Naphthalene
Petroleum - metabolism
Phenanthrene
Polycyclic aromatic hydrocarbons
Polycyclic Aromatic Hydrocarbons - metabolism
Polysaccharides, Bacterial - biosynthesis
Polysaccharides, Bacterial - pharmacology
Spain
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Summary:Halomonas eurihalina strains F2-7, H28, H96, H212 and H214 were capable of producing large amounts of exopolysaccharides (EPS) in MY medium with added crude oil. The biopolymers showed lower carbohydrate and protein content than those synthesised in control medium without oil. Nevertheless, the percentages of uronic acids, acetyls and sulphates were higher. The emulsifying activity of biopolymers was measured; crude oil was the substrate most efficiently emulsified. Furthermore, all the EPS tested emulsified higher volumes of crude oil than the commercial surfactants used as controls. We have also proved the effectiveness of both Halomonas eurihalina strains and their EPS to select indigenous bacteria able to grow in the presence of polycyclic aromatic hydrocarbons (naphthalene, phenanthrene and pyrene) from waste crude oil. The majority of isolated strains belonged to the genus Bacillus.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-002-1115-4