A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro

In this paper we describe the results obtained with a novel method to prepare depositions of asbestos fibres for toxicological tests . The technique is based on a micro-dispenser, working as an inkjet printer, able to deposit micro-sized droplets from a suspension of fibres in a liquid medium; we us...

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Published inFrontiers in chemistry Vol. 11; p. 1116463
Main Authors Della Ventura, Giancarlo, Rabiee, Ahmad, Marcelli, Augusto, Macis, Salvatore, D'Arco, Annalisa, Iezzi, Gianluca, Radica, Francesco, Lucci, Federico
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 14.02.2023
Subjects
asbestos fibres
Chemistry
deposition
image processing
microdrop method
optical and SEM images
toxicological experiments
image processing
deposition
asbestos fibres
optical and SEM images
microdrop method
toxicological experiments
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Summary:In this paper we describe the results obtained with a novel method to prepare depositions of asbestos fibres for toxicological tests . The technique is based on a micro-dispenser, working as an inkjet printer, able to deposit micro-sized droplets from a suspension of fibres in a liquid medium; we used here a highly evaporating liquid (ethanol) to reduce the experimental time, however other solvents could be used. Both the amount and spatial distribution of fibres on the substrate can be controlled by adjusting the parameters of the micro-dispenser such as deposition area, deposition time, uniformity and volume of the deposited liquid. Statistical analysis of images obtained by optical and scanning electron microscopy shows that this technique produces an extremely homogeneous distribution of fibers. Specifically, the number of deposited single fibres is maximized (up to 20 times), a feature that is essential when performing viability tests where agglomerated or untangled fibrous particles need to be avoided.
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This article was submitted to Inorganic Chemistry, a section of the journal Frontiers in Chemistry
Elena Belluso, University of Turin, Italy
Reviewed by: Riccardo Leinardi, Université catholique de Louvain, Belgium
Edited by: Jasmine Rita Petriglieri, University of Turin, Italy
Dario Di Giuseppe, University of Modena and Reggio Emilia, Italy
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2023.1116463