Possible role of hydrogen sulfide as an endogenous relaxation factor in the rat bladder and prostate

• Published in: Neurourology and urodynamics Vol. 37; no. 8; pp. 2519 - 2526
• Main Authors: Zou, Suo, Shimizu, Takahiro, Shimizu, Shogo, Higashi, Youichirou, Nakamura, Kumiko, Ono, Hideaki, Aratake, Takaaki, Saito, Motoaki
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.11.2018
• Subjects: Amino acid oxidase; Amino acids; Bladder; Carbachol; Enzymes; Epithelium; Ethyl carbamate; Hydrogen sulfide; Immunohistochemistry; Methylene blue; micturition; Muscle contraction; Norepinephrine; Propranolol; Prostate; Rodents; Smooth muscle; smooth muscle relaxation; Sulfurtransferase; Urination; Urothelium; smooth muscle relaxation; prostate; hydrogen sulfide; micturition; bladder
• ISSN: 0733-2467; 1520-6777
• DOI: 10.1002/nau.23788

Aims To clarify the roles of hydrogen sulfide (H2S), an endogenous gasotransmitter, in the rat bladder and prostate, we investigated the distribution of enzymes related to H2S biosynthesis (cystathionine β‐synthase [CBS], cystathionine γ‐lyase [CSE], 3‐mercaptopyruvate sulfurtransferase [MPST], cysteine aminotransferase [CAT], and D‐amino acid oxidase [DAO]) and the content of H2S. We also investigated the effects of H2S donors (NaHS and GYY4137) on the contractility of both tissues and on micturition. Methods The distribution of these enzymes was investigated by real‐time PCR, Western blot, and immunohistochemistry. Tissue H2S content was measured by the methylene blue method. The effects of NaHS (1 × 10−9 to 3 × 10−4 M) were evaluated on carbachol (10−5 M)‐induced pre‐contracted bladder strips, and on noradrenaline (10−5 M)‐induced pre‐contracted prostate strips, which were pretreated with propranolol (10−6 M). In addition, in urethane‐anesthetized male Wistar rats, the effects of intravesically instilled GYY4137 (10−8, 10−7, and 10−6 M) on micturition were evaluated by cystometry. Results MPST and CAT were detected in the bladder and prostate, CBS was only detected in the prostate, while CSE and DAO were not detected in both tissues. Immunoreactivity of these enzymes was mainly detected in the urothelium and smooth muscle layer of the bladder and in the prostate glandular epithelium. H2S was detected in both tissues. NaHS dose‐dependently induced relaxation of pre‐contracted bladder and prostate strips. Intravesically instilled GYY4137 significantly prolonged intercontraction intervals. Conclusions It is possible that H2S can function as an endogenous relaxation factor in the rat bladder and prostate.