Ten‐m/Odz3 regulates migration and differentiation of chondrogenic ATDC5 cells via RhoA‐mediated actin reorganization

• Published in: Journal of cellular physiology Vol. 236; no. 4; pp. 2906 - 2919
• Main Authors: Takano, Ikuko, Takeshita, Nobuo, Yoshida, Michiko, Seki, Daisuke, Oyanagi, Toshihito, Kimura, Seiji, Jiang, Wei, Sasaki, Kiyo, Sogi, Chisumi, Kawatsu, Masayoshi, Takano‐Yamamoto, Teruko
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2021
• Subjects: Actin; actin cytoskeleton; Actin Cytoskeleton - drug effects; Actin Cytoskeleton - metabolism; Actins - metabolism; Animals; ATDC5; Cartilage; Cell differentiation; Cell Differentiation - genetics; Cell Line; Cell Movement - genetics; Cell Proliferation; Cell Shape; Cell size; Chondrocytes - cytology; Chondrocytes - metabolism; Chondrogenesis; Chondrogenesis - genetics; Collagen (type II); Cytoskeleton; Fibroblast growth factor 2; Fibroblast Growth Factor 2 - metabolism; Gene expression; Gene Expression Regulation; Homology; Humans; Limb buds; Membrane Proteins - metabolism; Mesenchyme; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins - metabolism; RhoA; rhoA GTP-Binding Protein - metabolism; RhoA protein; Sox9 protein; Tenascin; Ten‐m/Odz3; actin cytoskeleton; ATDC5; chondrogenesis; RhoA; Ten-m/Odz3
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.30058

Tenascin‐like molecule major (Ten‐m)/odd Oz (Odz), a type II transmembrane molecule, is well known to modulate neural development. We have reported that Ten‐m/Odz3 is expressed in cartilaginous tissues and cells. Actin cytoskeleton and its regulator ras homolog gene family member A (RhoA) are closely associated with chondrogenesis. The present study aimed to evaluate the function and molecular mechanism of Ten‐m/Odz3 during chondrogenesis, focusing on RhoA and the actin cytoskeleton. Ten‐m/Odz3 was expressed in precartilaginous condensing mesenchyme in mouse limb buds. Ten‐m/Odz3 knockdown in ATDC5 induced actin cytoskeleton reorganization and change of cell shape through modulation of RhoA activity and FGF2 expression. Ten‐m/Odz3 knockdown suppressed ATDC5 migration and expression of genes associated with chondrogenesis, such as Sox9 and type II collagen, via RhoA. On the other hand, Ten‐m/Odz3 knockdown inhibited proliferation of ATDC5 in a RhoA‐independent manner. These findings suggest that Ten‐m/Odz3 plays an important role in early chondrogenesis regulating RhoA‐mediated actin reorganization. The present study aimed to evaluate the function and molecular mechanism of tenascin‐like molecule major (Ten‐m/Odz3) during chondrogenesis, focusing on ras homolog gene family member A (RhoA) and the actin cytoskeleton. Ten‐m/Odz3 most likely plays an important role in early chondrogenesis regulating RhoA‐mediated actin reorganization.