The effects of glucosamine hydrochloride on subchondral bone changes in an animal model of osteoarthritis

• Published in: Arthritis and rheumatism Vol. 56; no. 5; pp. 1537 - 1548
• Main Authors: Wang, Susanne X., Laverty, Sheila, Dumitriu, Mircea, Plaas, Anna, Grynpas, Marc D.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2007; Wiley
• Subjects: Absorptiometry, Photon; Administration, Oral; Animals; Anterior Cruciate Ligament - surgery; Biological and medical sciences; Bone and Bones - drug effects; Bone and Bones - metabolism; Bone and Bones - pathology; Bone Density - drug effects; Bones, joints and connective tissue. Antiinflammatory agents; Disease Models, Animal; Diseases of the osteoarticular system; Dose-Response Relationship, Drug; Glucosamine - administration & dosage; Glucosamine - pharmacology; Glucosamine - therapeutic use; Male; Medical sciences; Miscellaneous. Osteoarticular involvement in other diseases; Osteoarthritis; Osteoarthritis, Knee - drug therapy; Osteoarthritis, Knee - pathology; Osteogenesis - drug effects; Pharmacology. Drug treatments; Rabbits; Animal model; Femur; Glucosamine; Left; Diseases of the osteoarticular system; Rheumatology; Joint; Anterior cruciate ligament; Treatment; Articular cartilage; Hydrochlorides; Tibia; Arthropathy; Bone trabeculae; Degenerative disease; Bone mineral density; Antirheumatic agent; Bone; Osteoarthritis
• ISSN: 0004-3591; 1529-0131
• DOI: 10.1002/art.22574

Objective To quantify periarticular subchondral bone changes in a rabbit model of experimental osteoarthritis (OA), and to determine the effects of continuous administration of a clinically relevant dose of glucosamine HCl on subchondral bone changes in this model. Methods Anterior cruciate ligament transection (ACLT) was performed on the left femorotibial joints of 16 rabbits to induce OA. Ten rabbits that did not undergo ACLT served as unoperated controls. Eight rabbits that underwent ACLT and 6 control rabbits were treated with 100 mg of glucosamine daily, and the others were given a placebo. The articular cartilage was evaluated macroscopically and graded at the time of necropsy, 8 weeks after ACLT. Bone mineral density (BMD) was measured using dual‐energy x‐ray absorptiometry on the dissected distal femur and proximal tibia. Subchondral trabecular bone turnover, architecture, and connectivity, as well as subchondral plate thickness and mineralization were studied on the undecalcified tibia sections from each animal. Results Eight weeks after ACLT, most of the operated joints had various degrees of cartilage damage and fibrillation. Compared with the control group, the ACLT group had significantly increased subchondral bone turnover and lower BMD, bone volume, connectivity, and bone mineralization. The high bone turnover was significantly reduced in glucosamine‐treated animals that underwent ACLT. In fact, there were no significant differences between the ACLT/glucosamine group and the control/glucosamine group in most of the bone parameters studied. Conclusion This study shows that subchondral bone turnover, structure, and mineralization are significantly altered in the early stages of experimental OA, and that these changes are attenuated by glucosamine treatment.