Singlet Oxygen Luminescence Image in Blood Vessels During Vascular‐Targeted Photodynamic Therapy

• Published in: Photochemistry and photobiology Vol. 96; no. 3; pp. 646 - 651
• Main Authors: Lin, Lisheng, Lin, Huiyun, Shen, Yi, Chen, Defu, Gu, Ying, Wilson, Brian C., Li, Buhong
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.05.2020
• Subjects: Animals; Biological damage; Blood vessels; Blood Vessels - drug effects; Cytotoxicity; Field of view; Humans; In vivo methods and tests; Infrared imaging; Luminescence; Mice; Mice, Inbred BALB C; Photochemotherapy - methods; Photodynamic therapy; Photosensitizing Agents - pharmacology; Singlet oxygen; Singlet Oxygen - metabolism; Spatial discrimination; Spatial resolution; Vasoconstriction; Vasoconstriction - drug effects
• ISSN: 0031-8655; 1751-1097
• DOI: 10.1111/php.13264

Singlet oxygen (1O2) is widely regarded as the main cytotoxic substance that induces the biological damage for photodynamic therapy (PDT). In this study, the previously developed near‐infrared (NIR) optical imaging system was optimized for fast imaging of 1O2 luminescence. The optical imaging system enables direct imaging of 1O2 luminescence in blood vessels within 2 s during vascular‐targeted PDT (V‐PDT), which makes this system extremely practical for in vivo studies. The dependence of RB concentration on 1O2 luminescence image was investigated for V‐PDT, and the data imply that 1270 nm signal is attributed to 1O2 luminescence. The imaging system operates with a field of view of 9.60 × 7.68 mm2 and a spatial resolution of 30 μm, which holds the potential to elucidate the correlation between cumulative 1O2 luminescence and vasoconstriction for V‐PDT. Direct imaging of singlet oxygen luminescence in blood vessels in vivo was successfully achieved, which hold great promise in establishing the correlation between cumulative singlet oxygen luminescence and vasoconstriction for vascular‐targeted photodynamic therapy.