ABA‐responsive ABRE‐BINDING FACTOR3 activates DAM3 expression to promote bud dormancy in Asian pear

• Published in: Plant, cell and environment Vol. 43; no. 6; pp. 1360 - 1375
• Main Authors: Yang, Qinsong, Yang, Bo, Li, Jianzhao, Wang, Yan, Tao, Ruiyan, Yang, Feng, Wu, Xinyue, Yan, Xinhui, Ahmad, Mudassar, Shen, Jiaqi, Bai, Songling, Teng, Yuanwen
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.06.2020; Wiley Subscription Services, Inc
• Subjects: ABF3; Abscisic acid; Abscisic Acid - pharmacology; Binding; bud dormancy; Cell division; Cultivars; DAM; Dormancy; Flowers - physiology; Freezing; Gene expression; Gene Expression Regulation, Plant - drug effects; pear; Plant Dormancy - drug effects; Plant Proteins - genetics; Plant Proteins - metabolism; Promoter Regions, Genetic; Protein Binding - drug effects; Pyrus - genetics; Pyrus - physiology; Pyrus pyrifolia; Regulatory mechanisms (biology); Signal transduction; Signal Transduction - drug effects; Trans-Activators - metabolism; ABF3; Abscisic acid; DAM; pear; bud dormancy
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.13744

Bud dormancy is indispensable for the survival of perennial plants in cold winters. Abscisic acid (ABA) has essential functions influencing the endo‐dormancy status. Dormancy‐associated MADS‐box/SHORT VEGETATIVE PHASE‐like genes function downstream of the ABA signalling pathway to regulate bud dormancy. However, the regulation of DAM/SVP expression remains largely uncharacterized. In this study, we confirmed that endo‐dormancy maintenance and PpyDAM3 expression are controlled by the ABA content in pear (Pyrus pyrifolia) buds. The expression of pear ABRE‐BINDING FACTOR3 (PpyABF3) was positively correlated with PpyDAM3 expression. Furthermore, PpyABF3 directly bound to the second ABRE in the PpyDAM3 promoter to activate its expression. Interestingly, both PpyABF3 and PpyDAM3 repressed the cell division and growth of transgenic pear calli. Another ABA‐induced ABF protein, PpyABF2, physically interacted with PpyABF3 and disrupted the activation of the PpyDAM3 promoter by PpyABF3, indicating DAM expression was precisely controlled. Additionally, our results suggested that the differences in the PpyDAM3 promoter in two pear cultivars might be responsible for the diversity in the chilling requirements. In summary, our data clarify the finely tuned regulatory mechanism underlying the effect of ABA on DAM gene expression and provide new insights into ABA‐related bud dormancy regulation. ABA promotes bud dormancy via SVP/DAM genes but the underlying mechanism is unclear. A novel pear ABI5‐like ABA‐responsive bZIP transcription factor PpyABF3 directly and indirectly induces PpyDAM3 expression to promote bud dormancy. PpyABF2 interacts with PpyABF3 to disrupt the activation of PpyDAM3, suggesting DAM expression is precisely controlled.