Differentiation of Junin Virus and Antigenic Variants Isolated in vivo by Kinetic Neutralization Assays

• Published in: Journal of general virology Vol. 69; no. 8; pp. 2123 - 2127
• Main Authors: Alche, Laura E, Coto, Celia E
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.1988; Society for General Microbiology
• Subjects: Animals; Antibodies, Viral - immunology; Antigenic Variation; Antigens, Viral - analysis; Arenaviridae - isolation & purification; Arenaviruses, New World - immunology; Arenaviruses, New World - isolation & purification; Arvicolinae; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Hemorrhagic Fever, American - microbiology; Junin virus; Kinetics; Microbiology; Neutralization Tests; Techniques used in virology; Virology; Antibody; Rodentia; Kinetic method; Junin virus; Immunological method; Virus; Variant; Vertebrata; Mammalia; Neutralization test; Mouse; Newborn animal; Antigenic variation; Arenaviridae
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-69-8-2123

Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Piso 4, 1428-Buenos Aires, Argentina The major natural reservoir of Junin virus, the aetiological agent of Argentine haemorrhagic fever, is the cricetid Calomys musculinus . Neonatal animals experimentally infected with Junin virus (XJCl 3 strain) developed typical disease and approximately 80% of them died. Most survivors become persistently infected. Antigenically variant viruses were isolated from the blood and brain of infected cricetids during the acute and chronic stages of the disease. These variants could be distinguished from the parental strain by kinetic neutralization assays using polyclonal antibodies. Some biological properties were shared with the parental virus strain including its virulence for newborn C. musculinus . These variant viruses may play a major role in chronic disease since we have shown that a viral isolate from an infected brain was poorly neutralized by serum obtained from the same animal. Keywords: Junin virus, antigenic variants, kinetic neutralization assay Received 6 January 1988; accepted 5 May 1988.