Analysis of globin transition in Xenopus laevis and identification of globins by in vitro translation of hybrid-selected mRNA

• Published in: FEBS letters Vol. 205; no. 2; pp. 219 - 222
• Main Authors: Sandmeier, E., Gygi, D., Wyler, T., Nyffenegger, U., Weber, R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.09.1986; Elsevier
• Subjects: (Xenopus laevis) Globin Electrophoresis Hybrid selection Translation; (Xenopus laevis)GlobinElectrophoresisHybrid selectionTranslation; Applied sciences; Biological and medical sciences; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Molecular genetics; Other techniques and industries; Translation. Translation factors. Protein processing; Xenopus laevis; (Xenopus laevis) Globin Electrophoresis Hybrid selection Translation; Globin; Vertebrata; Messenger RNA; In vitro translation; Complementary DNA; Xenopus laevis; Amphibia; Salientia; Developmental stage; Metamorphosis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)80901-2

The switch from larval to adult globin synthesis during amphibian metamorphosis was analyzed by polyacrylamide gel electrophoresis in acid urea-Triton X-100. 4–6 slowly migrating components were detected in larvae and at least 4 faster components appear at metamorphosis and persist in the adult stage. To relate the globin subunits to the mRNA sequences, isolated as cDNA clones, mRNAs were hybrid-selected from total RNA of larval and adult erythrocytes and translated in a wheat germ system. The translation products were identified by comparison with the globin pattern of hemolysates