E‐cadherin‐dependent coordinated epithelial rotation on a two‐dimensional discoidal pattern

• Published in: Genes to cells : devoted to molecular & cellular mechanisms Vol. 28; no. 3; pp. 175 - 187
• Main Authors: Luo, Shuangyu, Furuya, Kanji, Matsuda, Kimiya, Tsukasa, Yuma, Usui, Tadao, Uemura, Tadashi
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.03.2023
• Subjects: 2D culture; Animals; Cadherins - metabolism; Cell Adhesion; Cell adhesion molecules; Cell migration; Cell Movement; cell–cell adhesion; collective cell migration; Dogs; Epithelial Cells - metabolism; E‐cadherin; Fibronectin; Madin Darby Canine Kidney Cells; MDCK II; rotation; cell-cell adhesion; 2D culture; collective cell migration; E-cadherin; MDCK II; rotation
• ISSN: 1356-9597; 1365-2443
• DOI: 10.1111/gtc.13001

In vivo, cells collectively migrate in a variety of developmental and pathological contexts. Coordinated epithelial rotation represents a unique type of collective cell migrations, which has been modeled in vitro under spatially confined conditions. Although it is known that the coordinated rotation depends on intercellular interactions, the contribution of E‐cadherin, a major cell–cell adhesion molecule, has not been directly addressed on two‐dimensional (2D) confined substrates. Here, using well‐controlled fibronectin‐coated surfaces, we tracked and compared the migratory behaviors of MDCK cells expressing or lacking E‐cadherin. We observed that wild‐type MDCK II cells exhibited persistent and coordinated rotations on discoidal patterns, while E‐cadherin knockout cells migrated in a less coordinated manner without large‐scale rotation. Our comparison of the collective dynamics between these two cell types revealed a series of changes in migratory behavior caused by the loss of E‐cadherin, including a decreased global migration speed, less regularity in quantified coordination, and increased average density of topological defects. Taken together, these data demonstrate that spontaneous initiation of collective epithelial rotations depends on E‐cadherin under 2D discoidal confinements.