Anti-Microbial Dendrimers against Multidrug-Resistant P. aeruginosa Enhance the Angiogenic Effect of Biological Burn-wound Bandages

• Published in: Scientific reports Vol. 6; no. 1; p. 22020
• Main Authors: Abdel-Sayed, Philippe, Kaeppeli, Ariane, Siriwardena, Thissa, Darbre, Tamis, Perron, Karl, Jafari, Paris, Reymond, Jean-Louis, Pioletti, Dominique P., Applegate, Lee Ann
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.02.2016; Nature Publishing Group
• Subjects: 13/107; 14/63; 631/1647/338/22; 631/61/2035; Angiogenesis; Angiogenesis Inducing Agents - pharmacology; Anti-Infective Agents - pharmacology; Bandages; Biological effects; Burn patients; Burns - complications; Cell Survival - drug effects; Dendrimers; Drug resistance; Fibroblasts - drug effects; Fibroblasts - metabolism; Growth factors; Humanities and Social Sciences; Humans; Medical dressings; Morbidity; multidisciplinary; Multidrug resistance; Multidrug resistant organisms; Neovascularization, Physiologic - drug effects; Nosocomial infection; Nosocomial infections; Polycations; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Science; Science (multidisciplinary); Skin; Tumor suppression; Vascularization; Wound Healing; Wound Infection - drug therapy; Wound Infection - microbiology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep22020

Multi-drug resistant Pseudomonas aeruginosa has increased progressively and impedes further regression in mortality in burn patients. Such wound infections serve as bacterial reservoir for nosocomial infections and are associated with significant morbidity and costs. Anti-microbial polycationic dendrimers G3KL and G3RL, able to kill multi-drug resistant P. aeruginosa, have been previously developed. The combination of these dendrimers with a class of biological bandages made of progenitor skin cells, which secrete growth factors, could positively impact wound-healing processes. However, polycations are known to be used as anti-angiogenic agents for tumor suppression. Since, neovascularization is pivotal in the healing of deep burn-wounds, the use of anti-microbial dendrimers may thus hinder the healing processes. Surprisingly, we have seen in this study that G3KL and G3RL dendrimers can have angiogenic effects. Moreover, we have shown that a dendrimer concentration ranging between 50 and 100 μg/mL in combination with the biological bandages can suppress bacterial growth without altering cell viability up to 5 days. These results show that antimicrobial dendrimers can be used in combination with biological bandages and could potentially improve the healing process with an enhanced angiogenesis.